PXD015149-1

PXD015149 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry detection of photodamage in human extracellular matrix assemblies
Description	Photoageing in skin is commonly recognised by architectural remodelling of dermal extracellular matrix components. Mass spectrometry was previously used to identify tissue-specific patterns of fibrillin-1 and collagen VI peptide spectrum matches (PXD008450). This study aimed to determine if the same mass spectrometry-based approach could detect peptide spectrum match patterns and significantly differences in relative abundance of peptide sequences characteristic of damage following exposure to UVR of co-purified suspensions of fibrillin and collagen VI microfibrils. Human dermal fibroblast-derived suspensions of microfibrils were irradiated with either broadband UVB or solar simulated radiation (SSR). UVR-induced molecular damage was characterised by proteolytic peptide generation with elastase followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). This allowed the molecular scale identification of UV-induced structural changes within two skin matrix assemblies. The proteomic approaches used have the potential to facilitate the rapid, protein-specific identification of differential molecular fingerprints of damage in key extracellular matrix proteins.
HostingRepository	PRIDE
AnnounceDate	2020-01-30
AnnouncementXML	Submission_2020-01-30_06:23:04.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Eckersley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 08:07:28	ID requested
⏵ 1	2020-01-30 06:23:05	announced

Dataset with its publication pending

submitter keyword: Human, microfibrils, collagen VI, fibrillin, LC-MS/MS, ultraviolet, ECM

Michael Sherratt
contact affiliation	Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK
contact email	michael.j.sherratt@manchester.ac.uk
lab head
Alexander Eckersley
contact affiliation	University of Manchester
contact email	alexander.eckersley@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015149
     1. Label: PRIDE project
     2. Name: Mass spectrometry detection of photodamage in human extracellular matrix assemblies