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PXD015149 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry detection of photodamage in human extracellular matrix assemblies
DescriptionPhotoageing in skin is commonly recognised by architectural remodelling of dermal extracellular matrix components. Mass spectrometry was previously used to identify tissue-specific patterns of fibrillin-1 and collagen VI peptide spectrum matches (PXD008450). This study aimed to determine if the same mass spectrometry-based approach could detect peptide spectrum match patterns and significantly differences in relative abundance of peptide sequences characteristic of damage following exposure to UVR of co-purified suspensions of fibrillin and collagen VI microfibrils. Human dermal fibroblast-derived suspensions of microfibrils were irradiated with either broadband UVB or solar simulated radiation (SSR). UVR-induced molecular damage was characterised by proteolytic peptide generation with elastase followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). This allowed the molecular scale identification of UV-induced structural changes within two skin matrix assemblies. The proteomic approaches used have the potential to facilitate the rapid, protein-specific identification of differential molecular fingerprints of damage in key extracellular matrix proteins.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Eckersley
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-23 08:07:28ID requested
12020-01-30 06:23:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, microfibrils, collagen VI, fibrillin, LC-MS/MS, ultraviolet, ECM
Contact List
Michael Sherratt
contact affiliationDivision of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK
contact emailmichael.j.sherratt@manchester.ac.uk
lab head
Alexander Eckersley
contact affiliationUniversity of Manchester
contact emailalexander.eckersley@manchester.ac.uk
dataset submitter
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Dataset FTP location
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