PXD015145-2

PXD015145 is an original dataset announced via ProteomeXchange.

Title	PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM RHEUMATOID ARTHRITIS SYNOVIAL FLUID
Description	Previously, extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators in recipient cells. However, mechanistic details are unclear and contaminants in EV enrichments might have compromised previous observations. We recently developed a novel, size exclusion chromatography (SEC)-based method of EV isolation capable of high-quality enrichments from human synovial fluid. Here, we employed this method in combination with high resolution mass spectrometry to accurately characterise the SF EV proteome and investigate contributions of SF EVs to inflammatory processes in RA.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:41.468.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 06:05:17	ID requested
1	2021-03-31 20:12:36	announced
⏵ 2	2024-10-22 05:20:43	announced	2024-10-22: Updated project metadata.

Foers AD, Dagley LF, Chatfield S, Webb AI, Cheng L, Hill AF, Wicks IP, Pang KC, Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid. Clin Transl Immunology, 9(11):e1185(2020) [pubmed]

10.1002/cti2.1185;

submitter keyword: Extracellular vesicles, label-free MS, rheumatoid arthritis

Ken C. Pang
contact affiliation	MCRI, Parkville, Australia
contact email	ken.pang@mcri.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD015145

PRIDE project URI

• PRIDE
  1. PXD015145
     1. Label: PRIDE project
     2. Name: PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM RHEUMATOID ARTHRITIS SYNOVIAL FLUID