PXD015082-2

PXD015082 is an original dataset announced via ProteomeXchange.

Title	identification of the endogenous insoluble host targets of the Salmonella effectors SSeK3
Description	Strains of Salmonella utilise two distinct type three secretion systems to deliver effector proteins directly into host cells. The Salmonella effector SseK3 is a arginine glycosyltransferase that modify mammalian proteins with N-acetyl glucosamine (GlcNAc). Here, we used Arg-GlcNAc glycopeptide immunoprecipitation and mass spectrometry appraoch to identify host proteins GlcNAcylated by endogenous levels of SseK3 during Salmonella infection. Focusing on the insoluble proteome we demostrate multiple typically insoluble proteins are targetted by SseK3 during infections. Surpisingly unlike SseK1 and the EPEC homologue NleB1 which target death domain contain proteins SseK3 appears to modify a number of proteins lacking death domains. These results demostrate that under endogenous infection non-death domain containing proteins can be modified by Salmonella.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:09:45.219.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nichollas Scott
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-20 01:49:54	ID requested
1	2020-07-27 23:50:35	announced
⏵ 2	2024-10-22 05:09:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: glycosylation,Salmonella, proteomics, Arg-GlcNAc, SseK3

Elizabeth L Hartland
contact affiliation	Centre for Innate Immunity and Infectious Diseases, HudsonInstitute of Medical Research, Clayton, Victoria, Australia
contact email	elizabeth.hartland@hudson.org.au
lab head
Nichollas Scott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015082

PRIDE project URI

• PRIDE
  1. PXD015082
     1. Label: PRIDE project
     2. Name: identification of the endogenous insoluble host targets of the Salmonella effectors SSeK3