PXD015081-2

PXD015081 is an original dataset announced via ProteomeXchange.

Title	The host exosome biogenesis pathway is essential for human cytomegalovirus virion assembly and egress
Description	Human Cytomegalovirus (HCMV) infects over 50% of humans, is a leading cause of congenital birth defects, and poses serious risks for immuno-compromised individuals. To expand the molecular knowledge governing virion assembly and egress, we initiated a proteomics-based investigation and identified a significant proportion of host exosome constituents in HCMV virions. Quantitative analysis of exosomes released from uninfected cells (765 proteins) revealed that over 99% of the protein cargo was subsequently integrated into virions, confirming near-complete acquisition of exosome biogenesis during infection. We confirmed VAMP3 is essential for viral trafficking and release of infectious progeny, further validating the intrinsic exploitation of the pathway by HCMV. Therefore, host exosomes serve as the molecular template for virion cargo addition, and provide an export mechanism for virion egress. Our discovery underpins future investigation of host exosome proteins as important modulators of viral maturation, and enhances the understanding of HCMV envelopment, trafficking, fusion and release.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:10.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rommel Mathias
SpeciesList	scientific name: Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5); NCBI TaxID: 10360; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-20 01:39:13	ID requested
1	2021-02-21 23:24:31	announced
⏵ 2	2024-10-22 05:19:14	announced	2024-10-22: Updated project metadata.

10.7554/elife.58288;

Turner DL, Korneev DV, Purdy JG, de Marco A, Mathias RA, The host exosome pathway underpins biogenesis of the human cytomegalovirus virion. Elife, 9():(2020) [pubmed]

submitter keyword: VAMP3, herpesvirus, proteomics, exosome, extracellular vesicle, CMV, viral infection, egress, virion assembly,Human cytomegalovirus

Rommel Mathias
contact affiliation	Infection and Immunity Program Biomedicine Discovery Institute Monash University 23 Innovation Walk Clayton VIC 3800 Australia
contact email	rommel.mathias@monash.edu
lab head
Rommel Mathias
contact affiliation	Monash University
contact email	rommel.mathias@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD015081

PRIDE project URI

• PRIDE
  1. PXD015081
     1. Label: PRIDE project
     2. Name: The host exosome biogenesis pathway is essential for human cytomegalovirus virion assembly and egress