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PXD015078-1

PXD015078 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGUN1 controls the accumulation of NEP-dependent transcripts in Arabidopsis cotyledons
DescriptionGUN1 proteins controls protein homeostasis in chloroplast development in cotyledons of the model plant Arabidopsis thaliana, via coordination of nuclear encoded polymerase (NEP)-dependent chloroplast genes expression with plastid encoded polymerase (PEP)-dependent chloroplast genes expression. Lack of GUN1 leads to development of abnormal plastids and, consequently, accumulation of nuclear-encoded chloroplast-targeted (NECT) proteins which in many cases have been found still in their precursor form. Data dependent acquisition (DDA) mass spectrometry analysis of cotyledons soluble fractions, as well as targeted proteomics analysis of specific FtsH protease forms recognized by FtsH antibodies in western blotting, have been performed to identify peptides from the chloroplast transit peptide (cTP) of NECT in cotyledons extracts of wild type and GUN1 knocked-out mutant plants. The aim was to compare the number of cTPs found in 6 days after sowing (DAS) seedlings grown on plates with or without the inhibitor of plastid translation lincomycin.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:37:35.026.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD015078
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrea Trotta
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListPhospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-19 07:36:53ID requested
12024-10-08 11:37:35announced
Publication List
Tadini L, Peracchio C, Trotta A, Colombo M, Mancini I, Jeran N, Costa A, Faoro F, Marsoni M, Vannini C, Aro EM, Pesaresi P, GUN1 influences the accumulation of NEP-dependent transcripts and chloroplast protein import in Arabidopsis cotyledons upon perturbation of chloroplast protein homeostasis. Plant J, 101(5):1198-1220(2020) [pubmed]
10.6019/PXD015078;
10.1111/tpj.14585;
Keyword List
submitter keyword: Arabidopsis thaliana, single column, Q-exactive HF, cotyledons, chloroplast transit peptide (cTP), nuclear-encoded chloroplast-targeted, GUN1 protein
Contact List
Eva-Mari Aro
contact affiliationMolecular Plant Biology, Department of Biochemistry, University of Turku
contact emailevaaro@utu.fi
lab head
Andrea Trotta
contact affiliationInstitute of Biosciences and Bioresources, National Research Council of Italy, Sesto Fiorentino (FI)
contact emailandrea.trotta@utu.fi
dataset submitter
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Dataset FTP location
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