PXD015078-1

PXD015078 is an original dataset announced via ProteomeXchange.

Title	GUN1 controls the accumulation of NEP-dependent transcripts in Arabidopsis cotyledons
Description	GUN1 proteins controls protein homeostasis in chloroplast development in cotyledons of the model plant Arabidopsis thaliana, via coordination of nuclear encoded polymerase (NEP)-dependent chloroplast genes expression with plastid encoded polymerase (PEP)-dependent chloroplast genes expression. Lack of GUN1 leads to development of abnormal plastids and, consequently, accumulation of nuclear-encoded chloroplast-targeted (NECT) proteins which in many cases have been found still in their precursor form. Data dependent acquisition (DDA) mass spectrometry analysis of cotyledons soluble fractions, as well as targeted proteomics analysis of specific FtsH protease forms recognized by FtsH antibodies in western blotting, have been performed to identify peptides from the chloroplast transit peptide (cTP) of NECT in cotyledons extracts of wild type and GUN1 knocked-out mutant plants. The aim was to compare the number of cTPs found in 6 days after sowing (DAS) seedlings grown on plates with or without the inhibitor of plastid translation lincomycin.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:37:35.026.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015078
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Phospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-19 07:36:53	ID requested
⏵ 1	2024-10-08 11:37:35	announced

Tadini L, Peracchio C, Trotta A, Colombo M, Mancini I, Jeran N, Costa A, Faoro F, Marsoni M, Vannini C, Aro EM, Pesaresi P, GUN1 influences the accumulation of NEP-dependent transcripts and chloroplast protein import in Arabidopsis cotyledons upon perturbation of chloroplast protein homeostasis. Plant J, 101(5):1198-1220(2020) [pubmed]

10.6019/PXD015078;

10.1111/tpj.14585;

submitter keyword: Arabidopsis thaliana, single column, Q-exactive HF, cotyledons, chloroplast transit peptide (cTP), nuclear-encoded chloroplast-targeted, GUN1 protein

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	Institute of Biosciences and Bioresources, National Research Council of Italy, Sesto Fiorentino (FI)
contact email	andrea.trotta@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015078
     1. Label: PRIDE project
     2. Name: GUN1 controls the accumulation of NEP-dependent transcripts in Arabidopsis cotyledons