PXD015050-2

PXD015050 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic analyses of protein PKA catalytic-alpha-null mpkCCD cells and PKA catalytic-beta-null mpkCCD cells.
Description	Protein Kinase A (PKA) is a widely studied protein that has been viewed by most investigators as a single entity, although its catalytic subunits are coded in the genome by two separate genes, PKA catalytic alpha (Gene symbol: Prkaca) and PKA catalytic beta Gene symbol: Prkacb). At an amino-acid level, the two are 91.5 percent identical and the catalytic domains are virtually identical. (Footnote: A third entity PKA catalytic gamma, is not widely expressed and will not be considered here.) We have recently succeeded in using CRISPR-Cas9 to create disruptive mutations in both PKA genes (PKA double KO, or PKA dKO) in vasopressin-responsive kidney epithelial cells (mpkCCD cells). Here we carry out mass spectrometry based quantitative proteomics and phosphoproteomics separately in PKA catalytic-alpha and PKA catalytic-beta single knockouts address the issue of function difference between these two PKA catalytic subunits.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:06:30.906.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kavee Limbutara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-16 01:27:13	ID requested
1	2020-06-01 05:38:05	announced
⏵ 2	2024-10-22 05:06:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: deep learning, benchmarking, machine learning

submitter keyword: Phosphoproteomics, PKA catalytic subunit, Kidney,PKA, Mouse, Proteomics, Kinase, mpkCCD

Mark A. Knepper
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	knepperm@nhlbi.nih.gov
lab head
Kavee Limbutara
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	kavee.limbutara@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD015050

PRIDE project URI

• PRIDE
  1. PXD015050
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic analyses of protein PKA catalytic-alpha-null mpkCCD cells and PKA catalytic-beta-null mpkCCD cells.