PXD015031-1

PXD015031 is an original dataset announced via ProteomeXchange.

Title	Label-free Quantitative Acetylomics Revealed Toxoplasma gondii Genotype-Specific Acetylome Signatures
Description	Distinct genotypic and pathogenicity differences exist between strains of the opportunistic protozoan Toxoplasma gondii. The label-free quantitative acetylomics approach was applied to investigate the differences in the level of acetylation between RH strain, PRU strain and PYS strain of T. gondii. The results showed that lysine acetylation in RH strain was the largest (458 acetylated proteins) among the three strains. The PYS strain had the second-largest acetylome (188 acetylated proteins), whereas lysine acetylation in PRU strain (115 acetylated proteins) was the smallest. Motif analysis revealed four, three and four motifs enriched from lysine acetylation sequences in RH strain, PRU strain and PYS strain respectively, suggesting specificity of acetyltransferases in these strains. Our analysis also identified 15 DAPs (differentially expressed acetylated proteins), 3 DAPs and 26 DAPs in RH strain vs PRU strain, PRU strain vs PYS strain and PYS strain vs RH strain, respectively. Compared with PYS strain and PRU strain, histone acetyltransferase and glycyl-tRNA synthase had higher expression levels in RH strain, reflecting genotype-specific differences in stress tolerance. These findings provide novel insight into the acetylomic profiles of major genotypes of T. gondii and provide an important resource for further analysis of the roles of the acetylated parasite proteins in the regulation of cellular functions.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_15:37:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ze-Xiang Wang
SpeciesList	scientific name: Toxoplasma; NCBI TaxID: 5810;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-15 01:18:22	ID requested
⏵ 1	2020-05-26 15:37:14	announced
2	2024-10-22 05:05:53	announced	2024-10-22: Updated project metadata.

Wang ZX, Hu RS, Zhou CX, He JJ, M Elsheikha H, Zhu XQ, Genotype-Specific Acetylomic Signatures. Microorganisms, 7(11):(2019) [pubmed]

submitter keyword: Toxoplasma gondii

Genotype

Tachyzoite

Acetylation

Label-free

Xing-Quan Zhu
contact affiliation	Professor and Head, Department of Parasitology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046,The People's Republic of China
contact email	xingquanzhu1@hotmail.com
lab head
Ze-Xiang Wang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (LVRI, CAAS)
contact email	neau601@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015031
     1. Label: PRIDE project
     2. Name: Label-free Quantitative Acetylomics Revealed Toxoplasma gondii Genotype-Specific Acetylome Signatures