PXD015021 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Generation of macrophages that are profoundly pro-remyelinating through unexpected activation cues |
Description | Excessive inflammation within the central nervous system is injurious, but an immune response is also required for its repair. Macrophages are versatile cells that adopt different properties depending upon their microenvironment. Exposing macrophages to interleukin-4 and -13 (IL4/IL13) has incurred interest for their reparative properties. Unexpectedly, while macrophages exposed to the classic pro-inflammatory signals (interferon-γ/lipopolysaccharide, IFN/LPS) killed neurons and oligodendrocytes in culture, the addition of LPS to IL4/IL13-treated macrophages profoundly elevated IL10, repair metabolites (lactate, ornithine), glucose metabolism and the oligodendrocyte-trophic heparin-binding epidermal growth factor (HBEGF); cells did not display pro-inflammatory or neurotoxic features. In mice with spinal cord demyelination, locally-applied IL4/IL13 was insufficient to alter responses beyond controls; remarkably, the LPS/IL4/IL13-treated animals significantly increased lesional phagocytic macrophages/microglia, lactate and HBEGF levels, oligodendrogenesis and remyelination. We report a remarkably reparative state of macrophages that is unexpectedly generated by the integration of pro- (LPS) and anti- (IL4/IL13) inflammatory activation cues. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-06 |
AnnouncementXML | Submission_2019-09-09_02:48:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Antoine Dufour |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-14 01:45:47 | ID requested | |
⏵ 1 | 2019-09-09 02:48:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Macrophage, Multiple sclerosis, remyelinating |
Contact List
Antoine Dufour |
contact affiliation | University of Calgary, Department of Physiology and Pharmacology |
contact email | antoine.dufour@ucalgary.ca |
lab head | |
Antoine Dufour |
contact affiliation | University of Calgary Department of Physiology and Pharmacology |
contact email | antoine.dufour@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015021
- Label: PRIDE project
- Name: Generation of macrophages that are profoundly pro-remyelinating through unexpected activation cues