PXD014997-2

PXD014997 is an original dataset announced via ProteomeXchange.

Title	MS-based proteomic and phosphoproteomic characterization of the AML proteome
Description	Acute myeloid leukemia (AML) is a hematological cancer that affects mainly the elderly. Although complete remission (CR) is achieved for the majority of the patients after induction and consolidation therapies, nearly two-thirds relapse within a short interval. Therefore, understanding of the biological factors that determine relapse has become a major interest in clinical AML. In order to identify the proteins and their phosphorylated modifications involved in AML relapse, we performed a global proteome and phosphoproteome study by liquid chromatography tandem mass spectrometry (LC-MS/MS) with primary cells from 41 AML patients at time of diagnosis that were defined as RELAPSE or REL_FREE according to their relapse status after a 5-year clinical follow-up post diagnosis. Our findings showed that the diagnostic sample of patients relapsing had increased levels of RNA processing and decreased expression of V-ATPases proteins along with CDKs and CSK2 activities. LC-MS/MS-based results were further validated with cell proliferation assays using V-ATPase inhibitor bafilomycin A1, CSK2 inhibitor CX-4945, CDK4/6 inhibitor abemaciclib and CDK2/7/9 inhibitor SNS-032. Our study presents molecules that could predict AML relapse and direct new therapeutic strategies that might circumvent more aggressive AML episodes.
HostingRepository	PRIDE
AnnounceDate	2020-04-14
AnnouncementXML	Submission_2020-06-30_04:26:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elise Aasebo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-12 07:28:56	ID requested
1	2020-04-13 23:04:07	announced
⏵ 2	2020-06-30 04:26:52	announced	2020-06-30: Updated publication reference for PubMed record(s): 32192169.
3	2024-10-22 05:02:07	announced	2024-10-22: Updated project metadata.

Aaseb, ø E, Berven FS, Bartaula-Brevik S, Stokowy T, Hovland R, Vaudel M, D, ø, skeland SO, McCormack E, Batth TS, Olsen JV, Bruserud Ø, Selheim F, Hernandez-Valladares M, Proteome and Phosphoproteome Changes Associated with Prognosis in Acute Myeloid Leukemia. Cancers (Basel), 12(3):(2020) [pubmed]

submitter keyword: Acute myeloid leukemia, proteomics, phosphoproteomics, human AML primary cells, LC-MSMS, super-SILAC

Frode Steingrimsen Berven
contact affiliation	The Proteomics Facility of the University of Bergen (PROBE), The Department of Biomedicine, University of Bergen, Bergen, Norway
contact email	frode.berven@uib.no
lab head
Elise Aasebo
contact affiliation	PROBE
contact email	elise.aasebo@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014997

PRIDE project URI

• PRIDE
  1. PXD014997
     1. Label: PRIDE project
     2. Name: MS-based proteomic and phosphoproteomic characterization of the AML proteome