PXD014997 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MS-based proteomic and phosphoproteomic characterization of the AML proteome |
Description | Acute myeloid leukemia (AML) is a hematological cancer that affects mainly the elderly. Although complete remission (CR) is achieved for the majority of the patients after induction and consolidation therapies, nearly two-thirds relapse within a short interval. Therefore, understanding of the biological factors that determine relapse has become a major interest in clinical AML. In order to identify the proteins and their phosphorylated modifications involved in AML relapse, we performed a global proteome and phosphoproteome study by liquid chromatography tandem mass spectrometry (LC-MS/MS) with primary cells from 41 AML patients at time of diagnosis that were defined as RELAPSE or REL_FREE according to their relapse status after a 5-year clinical follow-up post diagnosis. Our findings showed that the diagnostic sample of patients relapsing had increased levels of RNA processing and decreased expression of V-ATPases proteins along with CDKs and CSK2 activities. LC-MS/MS-based results were further validated with cell proliferation assays using V-ATPase inhibitor bafilomycin A1, CSK2 inhibitor CX-4945, CDK4/6 inhibitor abemaciclib and CDK2/7/9 inhibitor SNS-032. Our study presents molecules that could predict AML relapse and direct new therapeutic strategies that might circumvent more aggressive AML episodes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-14 |
AnnouncementXML | Submission_2020-06-30_04:26:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elise Aasebo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-12 07:28:56 | ID requested | |
1 | 2020-04-13 23:04:07 | announced | |
⏵ 2 | 2020-06-30 04:26:52 | announced | 2020-06-30: Updated publication reference for PubMed record(s): 32192169. |
3 | 2024-10-22 05:02:07 | announced | 2024-10-22: Updated project metadata. |
Publication List
Aaseb, ø E, Berven FS, Bartaula-Brevik S, Stokowy T, Hovland R, Vaudel M, D, ø, skeland SO, McCormack E, Batth TS, Olsen JV, Bruserud Ø, Selheim F, Hernandez-Valladares M, Proteome and Phosphoproteome Changes Associated with Prognosis in Acute Myeloid Leukemia. Cancers (Basel), 12(3):(2020) [pubmed] |
Keyword List
submitter keyword: Acute myeloid leukemia, proteomics, phosphoproteomics, human AML primary cells, LC-MSMS, super-SILAC |
Contact List
Frode Steingrimsen Berven |
contact affiliation | The Proteomics Facility of the University of Bergen (PROBE), The Department of Biomedicine, University of Bergen, Bergen, Norway |
contact email | frode.berven@uib.no |
lab head | |
Elise Aasebo |
contact affiliation | PROBE |
contact email | elise.aasebo@uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014997
- Label: PRIDE project
- Name: MS-based proteomic and phosphoproteomic characterization of the AML proteome