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PXD014983-1

PXD014983 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA technology to capture DNA binding proteins based on biolistic transformation
DescriptionInteractions between DNA and proteins are critical to almost all cellular functions, and the identification of proteins that bind to a DNA sequence of interest (gene-centered approach) is a widely studied area. However, a limited number of gene-centered methods have been developed. In the present study, we built a protein capture technology based on biolistic transformation (PCaT) to capture proteins bound by a target DNA. A promoter sequence or a DNA fragment was labeled with biotin and introduced into the host plants using biolistic transformation. The DNA and its bound proteins were cross-linked using formaldehyde in the transformed plants, and the labeled DNAs were captured using streptavidin magnetic beads, which enabled harvesting of the DNA-bound proteins. After mass spectrum analysis, the proteins associated with the target DNA were identified. Using this method, we identified the upstream regulators of a WRKY gene from Populus davidianaƗP. bolleana. The reliability of the identification of the upstream regulators of WRKY was confirmed using chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, the results showed PCaT provides a novel DNA and protein interactome discovery strategy. In addition, based on PCaT, a quick procedure to determine the upstream regulators of a gene of interest was proposed.
HostingRepositoryiProX
AnnounceDate2019-08-12
AnnouncementXMLSubmission_2019-08-12_02:15:11.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXin Zhang
SpeciesList scientific name: Populus davidiana x Populus alba var. pyramidalis; NCBI TaxID: 1550601;
ModificationListnatural residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-12 02:13:29ID requested
12019-08-12 02:15:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Biolistic transformation, biotin, DNA binding proteins, gene-centered approach, mass spectrum, streptavidin magnetic beads
Contact List
Chuanping Yang
contact affiliationState Key Laboratory of Tree Genetics and Breeding
contact emailgoodxin2016@163.com
lab head
Xin Zhang
contact affiliationState Key Laboratory of Tree Genetics and Breeding
contact emailgoodxin2016@nefu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI