PXD014967-2

PXD014967 is an original dataset announced via ProteomeXchange.

Title	Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regeneration Cells and buy Lung Proteomics
Description	During influenza pneumonia, the alveolar epithelial cells of the lungs are targeted by influenza virus. The distal airway stem cells (DASCs) and proliferating alveolar type II (AT2) cells are reported to be putative lung repair cells. However, their relative spatial and temporal distribution is still unknown during influenza-induced acute lung injury. Here, we investigated the distribution of these cells, and concurrently performed global proteomic analysis of the infected lungs to elucidate and link the cellular and molecular events during influenza pneumonia recovery. BALB/c mice were infected with a sub-lethal dose of influenza H1N1 virus. From 5 to 25 days post-infection (dpi), mouse lungs were subjected to histopathologic and immunofluorescence analysis to probe for global distribution of lung repair cells (using P63 and KRT5 markers for DASCs; PCNA and SPC markers for AT2 cells). At 7 and 15 dpi, infected mouse lungs were also subjected to protein mass spectrometry for relative protein quantification. DASCs appeared only in the damaged area of the lung from 7 dpi onwards, reaching a peak at 21 dpi, and persisted at 25 dpi. However, no differentiation of DASCs to AT2 cells was observed by 25 dpi. In contrast, AT2 cells began proliferating from 7 dpi to replenish its population. Mass spectrometry and gene ontology analysis revealed prominent innate immune response at 7 dpi, which shifted towards adaptive immune responses by 15 dpi. Hence, proliferating AT2 cells but not DASCs contribute to AT2 cell regeneration following transition from innate to adaptive immune responses during the early phase of recovery from influenza pneumonia up to 25 dpi.
HostingRepository	PRIDE
AnnounceDate	2019-09-30
AnnouncementXML	Submission_2019-10-01_01:16:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joe Ong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-09 01:31:28	ID requested
1	2019-09-30 05:42:12	announced
⏵ 2	2019-10-01 01:16:17	announced	2019-10-01: Updated publication reference for PubMed record(s): 31455003.
3	2019-11-27 06:53:06	announced	2019-11-27: Updated project metadata.

Ong JWJ, Tan KS, Ler SG, Gunaratne J, Choi H, Seet JE, Chow VT, Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regenerating Cells and by Lung Proteomics. Cells, 8(9):(2019) [pubmed]

submitter keyword: Influenza

Pneumonia

Lung Regeneration

Stem Cells

Comparative Quantification

P63

KRT5

Proliferating Alveolar Type 2 Cells

Proteomics

Vincent T. K. Chow
contact affiliation	Department: Department of Microbiology and Immunology Lab: Host and Pathogen Interactivity Lab Institute: National University of Singapore Country: Singapore
contact email	micctk@nus.edu.sg
lab head
Joe Ong
contact affiliation	National University of Singapore
contact email	micowjj@nus.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014967
     1. Label: PRIDE project
     2. Name: Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regeneration Cells and buy Lung Proteomics