PXD014958-1

PXD014958 is an original dataset announced via ProteomeXchange.

Title	Slow growing behavior in African trypanosomes during adipose tissue colonization
Description	When Trypanosoma brucei parasites, the causative agent of sleeping sickness, colonize the adipose tissue, they rewire gene expression. Whether this adaptation affects population behavior and disease treatment remained unknown. By using a mathematical model, we estimate that the population of adipose tissue forms (ATFs) proliferates slower than blood parasites. Analysis of the ATFs proteome, measurement of protein synthesis and proliferation rates confirm that the ATFs divide on average every 12hr, instead of 6hr in the blood. Importantly, the population of ATFs is heterogeneous with parasites doubling times ranging between 5hr and 35hr. Slow-proliferating parasites remain capable of reverting to the fast proliferation profile in blood conditions. Intravital imaging shows that ATFs are refractory to drug treatment. We propose that in adipose tissue, a subpopulation of T. brucei parasites acquire a slow growing behavior, which contributes to disease chronicity and treatment failure.
HostingRepository	PRIDE
AnnounceDate	2022-11-17
AnnouncementXML	Submission_2022-11-17_06:42:34.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FButter
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-08 06:24:50	ID requested
⏵ 1	2022-11-17 06:42:35	announced
2	2024-10-22 05:41:11	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: spatial proteomics

transcriptomics

gene annotation

Luisa MirandaFigueiredo
contact affiliation	Figueiredo Lab, Instituto de Medicina Molecular, Lisbon, Portugal
contact email	lmf@medicina.ulisboa.pt
lab head
FButter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD014958

PRIDE project URI

• PRIDE
  1. PXD014958
     1. Label: PRIDE project
     2. Name: Slow growing behavior in African trypanosomes during adipose tissue colonization