PXD014873-2

PXD014873 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum exported proteins co-immunoprecipitation LC-MS/MS
Description	The malaria parasite, Plasmodium falciparum, traffics the virulence protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) to the surface of infected red blood cells (RBCs) via membranous organelles known as the Maurer’s clefts. We developed a method for efficient enrichment of Maurer’s clefts and profiled the protein composition of this trafficking organelle. We identified 18 previously uncharacterised or poorly characterised Maurer’s cleft proteins. Transfectants expressing GFP-fusions of 7 of these proteins and confirmed their Maurer’s cleft location. Co-immunoprecipitation and mass spectrometry identified interacting partners of these Maurer’s clefts proteins, providing a network map of protein-protein associations. We identified two key clusters of proteins that may function in the loading and unloading of PfEMP1 into and out of the Maurer’s clefts.
HostingRepository	PRIDE
AnnounceDate	2020-02-20
AnnouncementXML	Submission_2020-03-24_04:37:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma McHugh
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-01 03:29:35	ID requested
1	2020-02-19 23:06:21	announced
⏵ 2	2020-03-24 04:37:52	announced	2020-03-24: Updated publication reference for PubMed record(s): 32184257.
3	2024-10-22 04:57:46	announced	2024-10-22: Updated project metadata.

McHugh E, Carmo OMS, Blanch A, Looker O, Liu B, Tiash S, Andrew D, Batinovic S, Low AJY, Cho HJ, McMillan P, Tilley L, Dixon MWA, Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking. mBio, 11(2):(2020) [pubmed]

submitter keyword: Plasmodium, LC-MS/MS, immunoprecipitation, Maurer's clefts

Matthew WA Dixon
contact affiliation	University of Melbourne
contact email	matthew.dixon@unimelb.edu.au
lab head
Emma McHugh
contact affiliation	University of Melbourne
contact email	mchugh.e@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014873

PRIDE project URI

• PRIDE
  1. PXD014873
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum exported proteins co-immunoprecipitation LC-MS/MS