PXD014857-2

PXD014857 is an original dataset announced via ProteomeXchange.

Title	The Drosophila CoREST interactome
Description	Affinity purification of dCoREST-interacting proteins from Drosophila melanogaster S2 cell nuclear extracts. An antibody recognizing both dCoREST isoforms was used to affinity purify proteins interacting with endogenous dCoREST from S2 nuclear extract. In addition, nuclear extracts from S2 cell lines ectopically expressing the FLAG-tagged dCoREST-L isoform and the FLAG-tagged dCoREST-M isoform, respectively, were subjected to anti-FLAG affinity purification to purify isoform specific dCoREST-interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:45.607.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-31 05:28:13	ID requested
1	2019-11-11 02:45:44	announced
⏵ 2	2024-10-22 04:58:46	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkz1050;

Ma, č, inkovi, ć I, Theofel I, Hundertmark T, Kova, č K, Awe S, Lenz J, Forn, é I, Lamp B, Nist A, Imhof A, Stiewe T, Renkawitz-Pohl R, Rathke C, Brehm A, Distinct CoREST complexes act in a cell-type-specific manner. Nucleic Acids Res, 47(22):11649-11666(2019) [pubmed]

submitter keyword: Endogenous dCoREST co-immunoprecipitation, FLAG-tagged dCoREST-L co-immunoprecipitation, FLAG-tagged dCoREST-M co-immunoprecipitation, dCoREST proteome

Prof. Dr. Alexander Brehm
contact affiliation	Institut für Molekularbiologie und Tumorforschung (IMT) Biomedizinisches Forschungszentrum Philipps-Universität Marburg Hans-Meerwein-Straße 2 35043 Marburg (Germany) Phone: +49-(0)6421-28-66840
contact email	brehm@imt.uni-marburg.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014857

PRIDE project URI

• PRIDE
  1. PXD014857
     1. Label: PRIDE project
     2. Name: The Drosophila CoREST interactome