PXD014844 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding Running Title: Proteins in tick saliva every 24 h during feeding |
Description | Amblyomma americanum ticks transmit more than a third of human tick-borne disease (TBD) agents in the United States. Tick saliva proteins are critical to success of ticks as vectors of TBD agents, and thus might serve as targets in tick antigen-based vaccines to prevent TBD infections. We describe a systems biology approach to identify, by LC-MS/MS, saliva proteins (tick=1182, rabbit=335) that A. americanum ticks likely inject into the host every 24 h during the first 8 days of feeding, and towards the end of feeding using two different sample preparation approaches (in-gel and in-solution). While the in-gel approach provided a link between the protein size and identification, the in-solution approach provided more depth in identifying an extensive list of proteins. Putative annotation grouped tick and rabbit proteins in A. americanum tick saliva into 27 and 25 functional categories, respectively. Similar to Prostriata Ixodes scapularis ticks, the most predominant functional category of proteins in A. americanum is tick-specific (32%). Other notable abundant saliva protein categories include protease inhibitors (13%), proteases (8%), glycine-rich proteins (6%) and lipocalins (4%). This study reveals several biologically relevant protein categories present in tick saliva that are important to tick feeding physiology and regulation of tick and host interactions, and highlights the potential role(s) of these proteins in evading host defense mechanisms. Of significance, 284 A. americanum tick saliva proteins are also secreted in saliva of other ticks and might represent those that regulate key tick feeding functions. We discuss our findings in the context of A. americanum tick feeding physiology. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-23 |
AnnouncementXML | Submission_2020-01-23_00:15:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Albert Mulenga |
SpeciesList | scientific name: Amblyomma americanum; NCBI TaxID: 6943; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-31 01:23:32 | ID requested | |
⏵ 1 | 2020-01-23 00:17:30 | announced | |
2 | 2020-01-23 01:57:31 | announced | 2020-01-23: Updated publication reference for DOI(s): 10.1101/763029. |
3 | 2023-11-14 08:50:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ticks, saliva, proteome, tick-feeding |
Contact List
Albert Mulenga |
contact affiliation | Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX, USA |
contact email | amulenga@cvm.tamu.edu |
lab head | |
Albert Mulenga |
contact affiliation | Texas A&M University |
contact email | mulengalab06@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014844 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014844
- Label: PRIDE project
- Name: Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding Running Title: Proteins in tick saliva every 24 h during feeding