PXD014807 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Domain model explains propagation dynamics and stability of K27 and K36 methylation landscape |
Description | Chromatin states must be stably maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications across cell division is thought to be central in this process. However, the histone modification landscape is challenged by the incorporation of new unmodified histones during each cell cycle and the principles that govern heritability remain poorly defined. Here, we take a quantitative approach and develop a reusable computational model that describes propagation of K27 and K36 methylation states. We measure combinatorial K27 and K36 methylation patterns by quantitative mass spectrometry on subsequent generations of histones in the presence and absence of enzymatic inhibition. Our modelling rejects active global demethylation and invoke the existence of 8 domains defined by distinct methylation endpoints. We find that K27me3 on pre- existing histones stimulates the rate of de novo K27me3 establishment, supporting a read-write mechanism in timely chromatin restoration. Finally, we provide a detailed, quantitative picture of the mutual antagonism between K27 and K37 methylation, and propose that this antagonism enhance the stability of epigenetic states across cell division. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:31:07.929.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Moritz Voelker-Albert |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monomethylated residue; 6x(13)C labeled residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-29 05:43:53 | ID requested | |
⏵ 1 | 2024-10-07 13:31:08 | announced | |
2 | 2024-10-22 04:58:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Q-Exactive HF, Chromatin domains,Histone modifications, Data modeling |
Contact List
Prof. Dr. Axel Imhof |
contact affiliation | Biomedical Center, Chromatin Proteomics Group, Department of Molecular Biology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Großhaderner Strasse 9, 82152 Planegg-Martinsried, Germany. |
contact email | imhof@lmu.de |
lab head | |
Moritz Voelker-Albert |
contact affiliation | LMU Munich |
contact email | Moritz.Voelker-Albert@med.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014807
- Label: PRIDE project
- Name: Domain model explains propagation dynamics and stability of K27 and K36 methylation landscape