PXD014797-2

PXD014797 is an original dataset announced via ProteomeXchange.

Title	Insight into the Human Pathodegradome of the V8 protease from Staphylococcus aureus
Description	The major human pathogen Staphylococcus aureus possesses ten extracellular proteases that are central to the virulence process. While some host targets of these and other bacterial proteases are known, a major bottleneck to the study of their function is the low-throughout nature of substrate identification. Rapid identification of proteolytic cleavage events in complex substrates has, however, been rendered possible by recent developments in the proteomics sub discipline of N-terminomics. Thus, herein we perform the first study of bacterial protease-host interactions, combining the S. aureus V8 protease and the infection relevant substrate human serum. In so doing, we identified ~90 host-protein targets of V8, including previously known and novel targets. Amongst these were complement components (C1r, C1s, C3,C4b, C4BPA, C5, C6, C8G, C9, CfB, CfH, and ficolin), iron sequestration and transport proteins (ceruloplasmin, serotransferrin, hemopexin, haptoglobin), clotting cascade proteins (plasminogen, kallikrein proteins, prothrombin, factors X and XII), and multiple host protease inhibitors (α2M and members of the ITIH and SERPIN families). N-terminomics also provided insight into the disruption of host protein function by highlighting specific target regions of host proteins, where V8 cleaved within peptidase and sushi domains of complement proteins, and, in the case of host protease inhibitors, cleavage occurred predominantly outside their protease-trapping motifs. Collectively, our data highlight the potential for further application of N-terminomics for the discovery of bacterial protease substrates in other host niches, and provides unique insight into the role of the V8 protease in S. aureus pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:34.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Frey
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: NCBITaxon:1280; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-homoarginine; biotinylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-29 02:00:07	ID requested
1	2021-03-29 11:33:49	announced
⏵ 2	2024-10-22 05:20:34	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: host-pathogen interactions, immunology, microbiology, N-terminomics,Staphylococcus aureus, degradomics

Lindsey Neil Shaw
contact affiliation	Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, Florida, USA
contact email	shaw@usf.edu
lab head
Andrew Frey
contact affiliation	University of South Florida
contact email	andrewfrey@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD014797

PRIDE project URI

• PRIDE
  1. PXD014797
     1. Label: PRIDE project
     2. Name: Insight into the Human Pathodegradome of the V8 protease from Staphylococcus aureus