PXD014760-1

PXD014760 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp. P. hybrida and D. stramonuim
Description	Floral nectar is a rich secretion produced by nectaries offered as an attractive reward to pollinators. Floral nectaries secrete an aqueous solution largely composed of sugars and amino acids as well as an array of proteins called nectarins. These provide one of the main mechanisms of floral defense in Nicotiana nectar. Due to its composition, floral nectar provides an ideal source of nutrients for specialized microorganisms that can change the nectar composition. Understanding of floral defenses and the role of nectar proteins is essential to predict the impacts in microbial growth, nectar composition, and relationships between plants and pollinators. In order to deepen knowledge about Nicotiana spp. nectar proteins, we used LC-MS/MS-based comparative proteomic analysis to identify 22 proteins from Petunia hybrida, 35 proteins from Datura stramonium, and 144 proteins from 23 different species of Nicotiana. GO enrichment analysis and secretory signal peptide prediction demonstrated that defense/stress was the largest group of proteins in nectars with differential abundance and distribution throughout genus Nicotiana. The Nicotiana spp. proteome consisted of 105 exclusive proteins such as lipid transfer proteins (LTPs), Nectar Redox Cycle proteins, proteases inhibitors (PI), and PR-proteins. Analysis by taxonomic sections demonstrated that LTPs were the most abundant group of proteins in the nectar of Undulatae and Noctiflora sections while nectarins were more abundant proteins in Rusticae, Suaveolens, Polydicliae, and Alata sections. Peroxidases (Pox) and chitinases (Chit) were exclusive to P. hybrida nectar, while D. stramonium proteome had only seven unique proteins. Significant differences were identified between the proteome of taxonomic sections providing relevant insights into the group of proteins related to defense/stress associated with Nectar Redox Cycle, antimicrobial proteins and signaling pathways. The activity of floral nectar proteins is suggested impact the microbial growth. The knowledge about these proteomes provides significant insights into the diversity of proteins secreted in the nectars and the array of mechanisms used by Nicotiana spp. in its floral defense.
HostingRepository	PRIDE
AnnounceDate	2020-01-08
AnnouncementXML	Submission_2020-01-07_16:13:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014760
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fredy Silva
SpeciesList	scientific name: Nicotiana forgetiana; NCBI TaxID: 118696; scientific name: Nicotiana langsdorffii; NCBI TaxID: 118700; scientific name: Nicotiana africana; NCBI TaxID: 200302; scientific name: Nicotiana x sanderae; NCBI TaxID: 200298; scientific name: Nicotiana paniculata; NCBI TaxID: 62141; scientific name: Nicotiana noctiflora; NCBI TaxID: 118705; scientific name: Nicotiana bonariensis; NCBI TaxID: 118694; scientific name: Nicotiana alata; NCBI TaxID: 4087; scientific name: Datura stramonium; NCBI TaxID: 4076; scientific name: Nicotiana quadrivalvis; NCBI TaxID: 278839; scientific name: Nicotiana glutinosa; NCBI TaxID: 35889; scientific name: Nicotiana plumbaginifolia; NCBI TaxID: 4092; scientific name: Nicotiana tabacum; NCBI TaxID: 4097; scientific name: Nicotiana longiflora; NCBI TaxID: 118702; scientific name: Nicotiana glauca; NCBI TaxID: 4090; scientific name: Nicotiana sylvestris; NCBI TaxID: 4096; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Nicotiana gossei; NCBI TaxID: 118698; scientific name: Nicotiana rustica; NCBI TaxID: 4093; scientific name: Nicotiana clevelandii; NCBI TaxID: 81866; scientific name: Nicotiana repanda; NCBI TaxID: 76336; scientific name: Nicotiana maritima; NCBI TaxID: 200308; scientific name: Nicotiana benavidesii; NCBI TaxID: 118691; scientific name: Petunia x hybrida; NCBI TaxID: 4102;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 03:36:50	ID requested
⏵ 1	2020-01-07 16:13:22	announced

Silva F, Guirgis A, von Aderkas P, Borchers CH, Thornburg R, LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp., Petunia hybrida and Datura stramonium. J Proteomics, 213():103618(2020) [pubmed]

submitter keyword: LC-MS/MS, Nicotiana, Nectar, Floral defense, Nectar Redox Cycle

Robert Thornburg
contact affiliation	Iowa State University - Department of Biochemistry Biophysics and Molecular Biology
contact email	thorn@iastate.edu
lab head
Fredy Silva
contact affiliation	Iowa State University
contact email	fredydavi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014760
     1. Label: PRIDE project
     2. Name: LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp. P. hybrida and D. stramonuim