PXD014756-1

PXD014756 is an original dataset announced via ProteomeXchange.

Title	Function of the MYND domain and C-terminal region in regulating the subcellular localization and catalytic activity of the SMYD family lysine methyltransferase Set5
Description	SMYD lysine methyltransferases target histones and non-histone proteins for methylation and are critical regulators of muscle developmental and implicated in neoplastic transformation. They are characterized by a split catalytic SET domain and an intervening MYND zinc finger domain, as well as an extended C-terminal domain. Saccharomyces cerevisiae contains two SMYD family members, Set5 and Set6, which share similar structural elements with the mammalian SMYD proteins. Set5 is a histone H4 lysine 5, 8, and 12 methyltransferase, implicated in the regulation of stress responses and genome stability. While the SMYD proteins have diverse roles in cells, there are many gaps in our understanding of how these enzymes are regulated. Here, we performed mutational analysis of Set5, combined with phosphoproteomics, to identify regulatory mechanisms for its enzymatic activity and subcellular localization. Our results indicate that the MYND domain promotes Set5 chromatin association in cells and mediates its interaction with DNA. Phosphoproteomics revealed extensive phosphorylation of Set5 and phosphomimetic mutations enhance Set5 catalytic activity and diminish its ability to interact with chromatin in cells. These studies uncover multiple regions within Set5 that regulate its localization and methyltransferase activity and highlight potential avenues for understanding mechanisms controlling the diverse roles of SMYD enzymes.
HostingRepository	PRIDE
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2019-11-11_02:41:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 02:57:04	ID requested
⏵ 1	2019-11-11 02:41:20	announced
2	2024-10-22 04:57:57	announced	2024-10-22: Updated project metadata.

Jaiswal D, Turniansky R, Moresco JJ, Ikram S, Ramaprasad G, Akinwole A, Wolf J, Yates JR, Green EM, Function of the MYND Domain and C-Terminal Region in Regulating the Subcellular Localization and Catalytic Activity of the SMYD Family Lysine Methyltransferase Set5. Mol Cell Biol, 40(2):(2020) [pubmed]

submitter keyword: methyltransferase, Saccharomyces cerevisiae

Erin. M. Green
contact affiliation	Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21230
contact email	egreen@umbc.edu
lab head
James Moresco
contact affiliation	The Scripps Research Institute
contact email	jmoresco@scripps.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014756
     1. Label: PRIDE project
     2. Name: Function of the MYND domain and C-terminal region in regulating the subcellular localization and catalytic activity of the SMYD family lysine methyltransferase Set5