PXD014675 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In Situ Structural Restraints from Crosslinking Mass Spectrometry in Human Mitochondria |
Description | The field of structural biology is increasingly focusing on studying proteins in situ, i.e. in a larger biological context. Crosslinking mass spectrometry is contributing to this effort, typically through the use of MS-cleavable crosslinkers. Here, we apply the popular non-cleavable crosslinker disuccinimidyl suberate to mitochondria and identify 5,518 distance restraints between protein residues. Each distance restraint within or between proteins provides structural information on proteins and their processes within mitochondria. Comparing these restraints to high-throughput comparative models and PDB deposited structures reveals novel protein conformations. Our data suggest substrates and flexibility of mitochondrial heat shock proteins. Crosslinking mass spectrometry is progressing towards large-scale in situ structural biology that reveals protein dynamics in addition to protein-protein interaction topologies. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-04 |
AnnouncementXML | Submission_2019-12-03_19:07:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Petra Ryl |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-19 04:28:02 | ID requested | |
⏵ 1 | 2019-12-03 19:07:22 | announced | |
Publication List
Ryl PSJ, Bohlke-Schneider M, Lenz S, Fischer L, Budzinski L, Stuiver M, Mendes MML, Sinn L, O'Reilly FJ, Rappsilber J, In Situ Structural Restraints from Cross-Linking Mass Spectrometry in Human Mitochondria. J Proteome Res, 19(1):327-336(2020) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: crosslinking mass spectrometry, in situ large-scale structural biology, non-cleavable DSS crosslinker, human mitochondria, comparative modelling |
Contact List
Juri Rappsilber |
contact affiliation | Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany |
contact email | juri.rappsilber@tu-berlin.de |
lab head | |
Petra Ryl |
contact affiliation | TU Berlin |
contact email | p.ryl@tu-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014675
- Label: PRIDE project
- Name: In Situ Structural Restraints from Crosslinking Mass Spectrometry in Human Mitochondria