PXD014669-1

PXD014669 is an original dataset announced via ProteomeXchange.

Title	Early proteomic responses in shoot and root of FL478 under salinity stress
Description	Climate change is affecting crop production due to soil salinization and water scarcity, and is predicted to worsen in the coming years. Rice is a major staple food and the most salt-sensitive cereal. High salinity in the soil triggers several adaptive responses in rice to cope with osmotic and ionic stress at the physiological, cellular and molecular levels. A major QTL for salinity tolerance, named Saltol, is present on chromosome 1 of Indian rice landrace varieties such as Pokkali and Nona Bokra. In this study, we characterized the physiological and early proteomic responses to salinity in FL478, an inbred rice line harboring the Saltol region. For this, plantlets were cultured in hydroponic cultures with 100 mM NaCl and evaluated at 6, 24 and 48h. At the physiological level, salinity significantly reduced shoot length after 48 h, whereas root length significantly increased. Moreover, the Na+/K+ ratio was maintained at lower levels in the shoots compared to the roots FL478 plantlets. On the other hand, roots showed a faster and more coordinated proteomic response than shoots, which was evident from only 6h of treatment. These responses were markedly related with transcription- and translation-related proteins. Moreover, roots exhibited a higher accumulation of stress-related proteins in response to salinity treatment, like peroxidase and SalT, which are both present in the Saltol QTL. Both, physiological and proteomic response, showed that roots respond in a highly adaptive manner to salinity stress compared to shoots, which suggests that this tissue is critical to the tolerance observed in varieties harbouring the Saltol region.
HostingRepository	PRIDE
AnnounceDate	2020-12-21
AnnouncementXML	Submission_2020-12-21_03:35:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014669
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Camilo López-Cristoffanini
SpeciesList	scientific name: Oryza sativa Indica Group; NCBI TaxID: 39946;
ModificationList	residues isobaric at 113.084064 Da; Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-19 01:05:11	ID requested
⏵ 1	2020-12-21 03:35:12	announced

Dataset with its publication pending

submitter keyword: FL478, Oryza sativa, rice, salinity, shoot, root, saoltol

Camilo López-Cristoffanini
contact affiliation	Department of Evolutionary Biology, Ecology and Environmental Sciences, Plant Physiology Section, University of Barcelone, 08028 Barcelone, Spain.
contact email	camilo.lopez.cr.puc@gmail.com
lab head
Camilo López-Cristoffanini
contact affiliation	Universidad de Barcelona
contact email	camilo.lopez.cr.puc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014669
     1. Label: PRIDE project
     2. Name: Early proteomic responses in shoot and root of FL478 under salinity stress