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PXD014644-1

PXD014644 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRole for ribosomal quality control in sampling proteins for MHC class I-mediated antigen presentation
DescriptionMammalian cells present a fingerprint of their proteome to the adaptive immune system through the display of endogenous peptides on MHC-I complexes. MHC-I peptides originate from protein degradation by the proteasome, suggesting that efficiently-folding, long-lived proteins could evade monitoring. Here, we investigate the role of the ribosomal quality control (RQC) pathway, responsible for the degradation of nascent chains stalled at the ribosome during translation, in sampling proteins for antigen presentation. We find that degradation and presentation on MHC-I of model proteins encoded by defective mRNAs is independent of their folding potential. Quantitative profiling of MHC-I peptides in wild-type and RQC-deficient cells by mass spectrometry showed that RQC substantially contributes to the composition of the immunopeptidome. Our results not only reveal the role of RQC in sampling proteins that are otherwise difficult to present due to their folding properties, but also identify endogenous substrates of the RQC pathway in human cells.
HostingRepositoryPRIDE
AnnounceDate2020-01-29
AnnouncementXMLSubmission_2020-01-29_04:50:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-17 04:05:47ID requested
12020-01-29 04:50:24announced
22024-10-22 03:58:55announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ribosomal quality control, Listerin, MHC-I, immunopeptidome
Contact List
F. Ulrich Hartl
contact affiliationDepartment of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
contact emailuhartl@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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