PXD014624 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanical forces regulate cardiomyocyte myofilament maturation via the VCL-SSH1-CFL axis |
Description | Mechanical forces regulate cell behavior and tissue morphogenesis. In particular, cardiac tissues require mechanical stimuli generated by the heartbeat for differentiation and maturation, but the molecular mechanisms underlying these processes remain unclear. Here, we first show that mechanical forces acting via the mechanosensitive factor Vinculin (VCL) are essential for cardiomyocyte myofilament maturation and that cardiac contractility regulates the localization and activation of Vinculin. To further analyze the role of Vinculin in myofilament maturation, we examined its interactome in contracting cardiomyocytes and found many cytoskeletal factors including actinins. We also identified Slingshot protein phosphatase 1 (SSH1), which we show is recruited by Vinculin to regulate F-actin rearrangement and myofilament maturation through its association with the actin depolymerizing factor Cofilin (CFL). Together, our results reveal that mechanical forces generated by cardiac contractility regulate cardiomyocyte maturation through the VCL-SSH1-CFL axis, providing mechanistic insight into how mechanical forces are transmitted intracellularly to regulate myofilament maturation. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-10 |
AnnouncementXML | Submission_2019-10-10_08:42:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Johannes Graumann |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-16 00:10:20 | ID requested | |
1 | 2019-09-06 02:14:02 | announced | |
⏵ 2 | 2019-10-10 08:42:47 | announced | 2019-10-10: Updated project metadata. |
3 | 2021-10-29 04:29:46 | announced | 2021-10-29: Updated project metadata. |
Publication List
Fukuda R, Gunawan F, Ramadass R, Beisaw A, Konzer A, Mullapudi ST, Gentile A, Maischein HM, Graumann J, Stainier DYR, Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis. Dev Cell, 51(1):62-77.e5(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Rattus norvegicus, cardiac contractility, heart |
Contact List
Didier Y.R. Stainier |
contact affiliation | Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany |
contact email | didier.stainier@mpi-bn.mpg.de |
lab head | |
Johannes Graumann |
contact affiliation | Max Planck Institute for Heart and Lung Research |
contact email | johannes.graumann@mpi-bn.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014624
- Label: PRIDE project
- Name: Mechanical forces regulate cardiomyocyte myofilament maturation via the VCL-SSH1-CFL axis