PXD014608 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PCT-assisted mass spectrometric quantification of gingival tissue reveals dynamics of protein synthesis during initiation and progression of inflammatory disease |
Description | Understanding the progression of periodontal destruction is at the forefront of periodontal research. Therefore, we aimed to capture the dynamics of gingival tissue proteome during initiation and progression of an experimental periodontitis developed by placing silk ligatures on the second molars of the specific pathogen-free C57BL/6 mice. Pressure cycling technology (PCT), a recently developed platform that uses ultra-high pressure to disrupt tissues, was utilized to achieve an efficient and reproducible protein extraction for quantitative shotgun proteomics. Lysis and protein digestion of gingival tissue was performed using PCT and analysed by Orbitrap Fusion. The proteomics data were quantitatively assessed with the Progenesis QI software. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-01-09_03:40:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kai Bao |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methionine oxidation with neutral loss of 64 Da; N-acylated residue; acetylated residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-15 02:16:04 | ID requested | |
⏵ 1 | 2020-01-09 03:40:05 | announced | |
Publication List
Bao K, Li X, Kajikawa T, Toshiharu A, Selevsek N, Grossmann J, Hajishengallis G, Bostanci N, Pressure Cycling Technology Assisted Mass Spectrometric Quantification of Gingival Tissue Reveals Proteome Dynamics during the Initiation and Progression of Inflammatory Periodontal Disease. Proteomics, 20(3-4):e1900253(2020) [pubmed] |
Keyword List
submitter keyword: Gingival tissue, experimental periodontitis, gingival inflammation, periodontitis, PCT, pressure cycling technology, proteome, label-free quantitation |
Contact List
Professor Nagihan Bostanci |
contact affiliation | Department of Dental Medicine (DENTMED), OF, Karolinska Institutet |
contact email | nagihan.bostanci@ki.se |
lab head | |
Kai Bao |
contact affiliation | postdoctoral researcher |
contact email | kai.bao@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014608 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014608
- Label: PRIDE project
- Name: PCT-assisted mass spectrometric quantification of gingival tissue reveals dynamics of protein synthesis during initiation and progression of inflammatory disease