PXD014574 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Large-Scale Profiling of Protein Methylation |
Description | Develop a novel de-glyco-assisted methylation site identification (DOMAIN) strategy which enables straightforward, fast, and reproducible analysis of protein methylation in a proteome-wide manner. Combining multidimensional fractionation and multiprotease digestion, our method enabled the identification of 573 methylated forms in 270 proteins, including 311 new methylation forms, in A549 cells. Combining this technique with stable isotope labeling quantitative proteomics and RNA interference, we determined the differential regulation of several putative methylated sites that are related to the protein arginine N-methyltransferase 3 (PRMT3). Collectively, our integrated proteomics workflow for comprehensive mapping of methylation sites enables a better understanding of protein methylation, while providing a rapid and effective approach for global protein methylation analysis in biomedical research. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-18 |
AnnouncementXML | Submission_2019-07-18_00:06:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Min Ma |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-11 07:41:36 | ID requested | |
⏵ 1 | 2019-07-18 00:23:17 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: methylation, hydrophilic, mutliprotease,LysargiNase |
Contact List
Lingjun Li |
contact affiliation | University of Wisconsin-Madison |
contact email | lingjun.li@wisc.edu |
lab head | |
Min Ma |
contact affiliation | UW madison |
contact email | mma58@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014574
- Label: PRIDE project
- Name: Large-Scale Profiling of Protein Methylation