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PXD014560-1

PXD014560 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic data of MG132 treated A. thaliana WT and ppi2
DescriptionA mutation in the proteasomal lid subunit Rpn8a alters proteasome composition and exerts a beneficial effect on photosynthesis. The effect is particularly visible in a double mutant between rpn8a and the plastid protein import receptor Toc159 (ppi2xrpn8a) but also in an rpn8a single mutant that shows higher PSII efficiency and lower non-photochemical quenching. The double mutant shows elevated accumulation of pigments, higher levels of thylakoid membrane proteins and higher PSII quantum yield compared to the ppi2 single mutant. While proplastid-like pro-thylakoid structures are characteristic for ppi2, stacked thylakoids are frequently observed in the double mutant. The rpn8a mutation is accompanied by higher 20S and lower 26S proteasome abundance and increased expression levels of proteolytic stress markers. Unlike known retrograde signaling chains, there is no effect on the transcription of selected photosynthesis associated nuclear encoded gens (PhanGs). Since plastid precursor protein abundance is elevated in the double mutant it is conceivable that the changes in proteasome composition affect precursor stability. We propose a model in which mild proteasome impairment shifts the equilibrium between precursor import and degradation towards import, which is especially effective in plastid protein import mutants but also detectable in the wildtype background, albeit to a much weaker extent.
HostingRepositoryPRIDE
AnnounceDate2020-05-27
AnnouncementXMLSubmission_2020-05-26_17:39:52.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSacha Baginsky
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-10 08:59:57ID requested
12020-05-26 17:39:53announced
22024-10-22 05:06:24announced2024-10-22: Updated project metadata.
Publication List
Grimmer J, Helm S, Dobritzsch D, Hause G, Shema G, Zahedi RP, Baginsky S, Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts. Nat Commun, 11(1):1662(2020) [pubmed]
Keyword List
submitter keyword: ubiquitin proteasome system
plastid protein import
Contact List
Sacha Baginsky
contact affiliationInstitute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120 Halle (Saale), Germany
contact emailpflanzen@biochemtech.uni-halle.de
lab head
Sacha Baginsky
contact affiliationInstitut fuer Biochemie und Biotechnologie
contact emailpflanzen@biochemtech.uni-halle.de
dataset submitter
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Dataset FTP location
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