PXD014555 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A PhotoClick cholesterol-based quantitative proteomics screen for cytoplasmic sterol binding proteins in Saccharomyces cerevisiae |
Description | Ergosterol is a prominent component of the yeast plasma membrane and essential for yeast cell viability. It is synthesized in the endoplasmic reticulum and transported to the plasma membrane by non-vesicular mechanisms requiring carrier proteins. Oxysterol binding protein homologues and yeast StARkin proteins have been proposed to function as sterol carriers. Although many of these proteins are capable of transporting sterols between synthetic lipid vesicles in vitro, they are not essential for ergosterol transport in cells, indicating that they may be functionally redundant with each other or with additional - as yet unidentified - sterol carriers. To address this point we hypothesized that sterol transport proteins are also sterol binding proteins (SBPs), and used an in vitro chemo-proteomic strategy to identify all cytosolic SBPs. We generated a cytosol fraction enriched in SBPs and captured the proteins with a photoreactive clickable cholesterol analog. Quantitative proteomics of the captured proteins identified 342 putative SBPs. Analysis of these identified proteins based on their annotated function, reported drug phenotypes, interactions with proteins regulating lipid metabolism, gene ontology, and presence of mammalian orthologs revealed a subset of 62 characterized and nine uncharacterized candidates. Five of the uncharacterized proteins play a role in maintaining plasma membrane integrity as their absence affects the ability of cells to grow in the presence of nystatin or myriocin. We anticipate that the dataset reported here will be a comprehensive resource for functional analysis of sterol binding/transport proteins and provide insights into novel aspects of non-vesicular sterol trafficking. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-27 |
AnnouncementXML | Submission_2019-11-27_06:01:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Johannes Graumann |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-10 03:23:46 | ID requested | |
1 | 2019-11-25 06:27:54 | announced | |
⏵ 2 | 2019-11-27 06:01:03 | announced | 2019-11-27: Updated project metadata. |
3 | 2021-10-29 04:31:19 | announced | 2021-10-29: Updated project metadata. |
Publication List
Chauhan N, Sere YY, Sokol AM, Graumann J, Menon AK, A PhotoClick cholesterol-based quantitative proteomics screen for cytoplasmic sterol-binding proteins in Saccharomyces cerevisiae. Yeast, 37(1):15-25(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: LC-MS2, sterol, chemical biology |
Contact List
Anant K. Menon |
contact affiliation | Weill Cornell Medical College, New York, NY |
contact email | akm2003@med.cornell.edu |
lab head | |
Johannes Graumann |
contact affiliation | Max Planck Institute for Heart and Lung Research |
contact email | johannes.graumann@mpi-bn.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014555
- Label: PRIDE project
- Name: A PhotoClick cholesterol-based quantitative proteomics screen for cytoplasmic sterol binding proteins in Saccharomyces cerevisiae