PXD014545-1

PXD014545 is an original dataset announced via ProteomeXchange.

Title	mTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Description	Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-based quantification method using non-isobaric TMT variants: TMT0 (TMTzero) and shTMT (super-heavy TMT). We term this strategy mTMT (mass difference tandem mass tagging), as these TMT variants differ by 11 mass units; yet, peptides labeled with these reagents co-elute, analogous to SILAC-labeled sample analysis. As proof-of-concept, we profiled the proteomes of two cell lines that are frequently used in neuroscience studies, SH-SY5Y and SVGp12, using mTMT and standard SILAC-labeling approaches. We show similar numbers of quantified proteins and peptides using each method with highly correlated fold changes between workflows. We conclude that mTMT is a suitable alternative for precursor-based protein quantification.
HostingRepository	PRIDE
AnnounceDate	2019-09-16
AnnouncementXML	Submission_2019-09-16_00:59:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-10 01:43:22	ID requested
⏵ 1	2019-09-16 00:59:45	announced
2	2024-10-22 04:04:08	announced	2024-10-22: Updated project metadata.

Paulo JA, Gygi SP, mTMT: An Alternative, Nonisobaric, Tandem Mass Tag Allowing for Precursor-Based Quantification. Anal Chem, 91(19):12167-12172(2019) [pubmed]

submitter keyword: TMT, isobaric, metabolic, SILAC, SH-SY5Y, SVGp12

Joao A. Paulo
contact affiliation	Joao Paulo Cell Biology Harvard Medical School USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014545
     1. Label: PRIDE project
     2. Name: mTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification