PXD014476-1
PXD014476 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A reference map of the human proinsulin biosynthetic interaction network |
Description | A monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma. Immunoprecipitated proinsulin or control IgG immunoprecipitates from human islets were then subjected to state of the art mass spectrometry revealing a rich proinsulin biosynthetic network that is remarkably conserved across a diverse group of donors. |
HostingRepository | MassIVE |
AnnounceDate | 2019-07-03 |
AnnouncementXML | Submission_2019-07-03_17:48:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre Rosa Campos |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation; TMT6plex |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-07-03 15:25:44 | ID requested | |
⏵ 1 | 2019-07-03 17:48:56 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Diabetes, Type 2 Diabetes, Interactome, Proinsulin misfolding, Affinity Purification-Mass spectrometry |
Contact List
Pamela Itkin-Ansari | |
---|---|
contact affiliation | Sanford Burnham Prebys Medical Discovery Institute |
contact email | pitkin@sbpdiscovery.org |
lab head | |
Alexandre Rosa Campos | |
contact affiliation | SBP Medical Discovery Institute |
contact email | arosacampos@sbpdiscovery.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084057 |