PXD014453-1
PXD014453 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Revealing dynamic protein acetylation across subcellular compartments |
| Description | Protein acetylation is a widespread post-translational modification implicated in many cellular processes. Recent advances in mass spectrometry have enabled the cataloging of thousands of sites throughout the cell, however identifying regulatory acetylation marks has proven to be a daunting task. Knowledge of the kinetics and stoichiometry of site-specific acetylation are important factors to uncover function. Here, an improved method of quantifying acetylation stoichiometry was developed and validated, providing a detailed landscape of dynamic acetylation stoichiometry within cellular compartments. The dynamic nature of site-specific acetylation in response to serum stimulation was revealed. In two distinct human cell lines, growth factor stimulation led to site-specific, temporal acetylation changes, revealing diverse kinetic profiles that clustered into several groups. Overlap of dynamic acetylation sites among the two cell types suggested similar regulatory control points across major cellular pathways that include splicing, translation, and protein homeostasis. Rapid increases in acetylation on protein translational machinery suggests a positive regulatory role under pro-growth conditions. Lastly, higher median stoichiometry was observed in cellular compartments where active acetyltransferases are well-described. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-04-07 |
| AnnouncementXML | Submission_2020-04-07_12:31:05.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alexis Lawton |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-07-01 10:13:02 | ID requested | |
| ⏵ 1 | 2020-04-07 12:31:06 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Acetylation, Mass Spectrometry, Data independent analysis, Post-translational modifications, Time-course |
Contact List
| John Denu | |
|---|---|
| contact affiliation | University of Wisconsin - Madison |
| contact email | john.denu@wisc.edu |
| lab head | |
| Alexis Lawton | |
| contact affiliation | University of Wisconsin - Madison |
| contact email | alawton2@wisc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084029/ |
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