PXD014443-1

PXD014443 is an original dataset announced via ProteomeXchange.

Title	Interferon stimulated changes in the brain phosphoproteome
Description	Type I interferons (IFN-Is) are immunoregulatory cytokines that are essential for normal host antiviral responses. The current understanding is that IFN-Is mediate their effects through the expression of several hundred interferon-regulated genes. Here we identified a novel IFN-I response mechanism that relies on widespread changes in protein phosphorylation. IFN-I-induced phosphorylation in primary murine microglia and astrocytes – key IFN-I-responding cells of the central nervous system – was rapid and thus largely independent of gene expression. Based on in silico analysis, this mechanism relies predominantly on a small number of kinase families. Further, functional analysis suggested that this rapid response induces an immediate reactive state in cells and prepares them for the subsequent transcriptomic response. Similar extensive phosphoproteome changes were also present in a mouse model for IFN-I-induced neuroinflammatory diseases. Strikingly, the altered phosphoproteome in these transgenic mice predicted the clinical and pathological outcome of neuroinflammatory disease. These findings demonstrate for the first time a novel mechanism by which IFN-Is mediate cellular responses.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:09:00.411.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014443
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Melanie White
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ4plex; Phospho; Deamidated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 02:23:56	ID requested
⏵ 1	2024-10-08 11:09:00	announced

10.6019/PXD014443;

submitter keyword: astrocytes, brain,Mouse, interferon, microglia, phosphoproteomics, LC-MS/MS

Melanie Yvonne White
contact affiliation	Discipline of Pathology, Charles Perkins Centre, University of Sydney, Australia
contact email	melanie.white@sydney.edu.au
lab head
Melanie White
contact affiliation	University of Sydney
contact email	melanie.white@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014443
     1. Label: PRIDE project
     2. Name: Interferon stimulated changes in the brain phosphoproteome