PXD014441-2

PXD014441 is an original dataset announced via ProteomeXchange.

Title	CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks
Description	The pleiotropic CCCTC-binding factor (CTCF) plays a role in homologous recombination (HR) repair of DNA double-strand breaks (DSBs). However, the precise mechanistic role of CTCF in HR remains largely unclear. Here, we show that CTCF engages in DNA end resection, which is the initial, crucial step in HR, through its interactions with MRE11 and CtIP. Depletion of CTCF profoundly impairs HR and attenuates CtIP recruitment at DSBs. CTCF physically interacts with MRE11 and CtIP and promotes CtIP recruitment to sites of DNA damage. Subsequently, CTCF facilitates DNA end resection to allow HR, in conjunction with MRE11-CtIP. Notably, the zinc finger domain of CTCF binds to both MRE11 and CtIP and enables proficient CtIP recruitment, DNA end resection, and HR. The N-terminus of CTCF is able to bind to only MRE11 and its C-terminus is incapable of binding to MRE11 and CtIP, thereby resulting in compromised CtIP recruitment, DSB resection, and HR. Overall, this suggests an important function of CTCF in DNA end resection through the recruitment of CtIP at DSBs. Collectively, our findings identify a critical role of CTCF at the first control point in selecting the HR repair pathway
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:25.139.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Byung-gyu kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 01:40:19	ID requested
1	2019-11-11 17:07:36	announced
⏵ 2	2024-10-22 04:05:29	announced	2024-10-22: Updated project metadata.

Hwang SY, Kang MA, Baik CJ, Lee Y, Hang NT, Kim BG, Han JS, Jeong JH, Park D, Myung K, Lee JS, CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks. Nucleic Acids Res, 47(17):9160-9179(2019) [pubmed]

10.1093/nar/gkz639;

submitter keyword: pleiotropic CCCTC-binding factor ,MRE11, human, interactome

Jong-Soo Lee
contact affiliation	Department of Life Science, College of Natural Sciences, Ajou University, 206, Worldcup-ro, Yeongtong-gu, Suwon, Gyeoggi-do 16499, Korea
contact email	jsjlee@ajou.ac.kr
lab head
Byung-gyu kim
contact affiliation	Center for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact email	goldenlion@ibs.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014441

PRIDE project URI

• PRIDE
  1. PXD014441
     1. Label: PRIDE project
     2. Name: CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks