PXD014436 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Conserved GTPase LepA (Elongation Factor 4) functions in biogenesis of the 30S subunit of the 70S ribosome |
Description | The physiological role of LepA, a paralog of EF-G found in all bacteria, has been a mystery for decades. Here, we show that LepA functions in ribosome biogenesis. In cells lacking LepA, immature 30S particles accumulate. Four proteins are specifically underrepresented in these particles-S3, S10, S14, and S21-all of which bind late in the assembly process and contribute to the folding of the 3' domain of 16S rRNA. Processing of 16S rRNA is also delayed in the mutant strain, as indicated by increased levels of precursor 17S rRNA in assembly intermediates. Mutation ΔlepA confers a synthetic growth phenotype in absence of RsgA, another GTPase, well known to act in 30S subunit assembly. Analysis of the ΔrsgA strain reveals accumulation of intermediates that resemble those seen in the absence of LepA. These data suggest that RsgA and LepA play partially redundant roles to ensure efficient 30S assembly. This data is published in paper PMID: 28096346 |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-03 |
AnnouncementXML | Submission_2019-07-03_07:47:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jenny Moon |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-01 01:39:25 | ID requested | |
⏵ 1 | 2019-07-03 07:47:43 | announced | |
Publication List
Gibbs MR, Moon KM, Chen M, Balakrishnan R, Foster LJ, Fredrick K, Conserved GTPase LepA (Elongation Factor 4) functions in biogenesis of the 30S subunit of the 70S ribosome. Proc Natl Acad Sci U S A, 114(5):980-985(2017) [pubmed] |
Keyword List
submitter keyword: EF4 |
RsgA |
YjeQ |
protein synthesis |
translation |
Contact List
Leonard J. Foster |
contact affiliation | Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada |
contact email | foster@msl.ubc.ca |
lab head | |
Jenny Moon |
contact affiliation | University of British Columbia |
contact email | kyungmee@mail.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014436
- Label: PRIDE project
- Name: Conserved GTPase LepA (Elongation Factor 4) functions in biogenesis of the 30S subunit of the 70S ribosome