PXD014425-2

PXD014425 is an original dataset announced via ProteomeXchange.

Title	Silver and platinum nanoparticles synergy
Description	In this study, we compared the effects of silver and platinum NPs on the main components of the blood brain barrier model: human cerebral microvascular endothelial cells (hCMEM/D3) and human primary astrocytes under single and co-exposure NP conditions. The co-exposure to two types of NPs synergistically inhibited proliferation of both cell types with the greater extent observed for endothelial cells (the combination index (CI) values for all tested concentrations were below 0.2 corresponding to strong or extreme synergism). In addition, NP-induced toxicity demonstrated dependence on the cell type with astrocytes being more tolerant to the metal NPs. The mechanism of synergy was further explored with short-time incubation time points (up to 30 min), where the cell metabolic activity was decreased to approximately 60% of the controls. The data obtained from the proteomics analysis suggests immunosuppression and deregulation of the extracellular matrix organization as well as contribution of the p75NTR-associated cell death executor (NADE)-dependent apoptotic mechanism in the synergistic cytotoxicity of NPs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:17:42.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vladimir Gorshkov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-28 06:51:36	ID requested
1	2022-11-08 08:00:09	announced
⏵ 2	2023-11-14 08:17:43	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: platinum, astrocytes, blood brain barrier, endothelial cells,silver, nanoparticles, synergy, cytotoxicity

Frank Kjeldsen
contact affiliation	Professor Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55 DK-5230 Odense M Denmark
contact email	frankk@bmb.sdu.dk
lab head
Vladimir Gorshkov
contact affiliation	University of Southern Denmark
contact email	vgor@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD014425

PRIDE project URI

• PRIDE
  1. PXD014425
     1. Label: PRIDE project
     2. Name: Silver and platinum nanoparticles synergy