PXD014410-1

PXD014410 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of the multi functional signal transducer B-cell adaptor protein (BCAP) facilitates recruitment of multiple SH2/SH3 proteins including Grb2.
Description	B-cell adaptor protein (BCAP) is a multimodular, multi-functional signal transducer that regulates signal transduction processes in leukocytes including macrophages, B-cells and T-cells. In particular BCAP acts as a negative regulator of inflammatory signaling by the Toll-like receptors. Here we characterize the complex phosphorylation patterns of BCAP and use a novel protein complex trapping strategy (virotrap) to identify interaction partners. This analysis identifies known interactions with BCAP with PI3K p85 subunit and Nck, together with novel SH2 and SH3 domain adaptor proteins notably Grb2 and CRKL. We show that the SH3 domain of Grb2 can bind to BCAP independently of phosphorylation, suggesting that the SH2 domains mediate interaction with activated receptor tyrosine kinase complexes including the CD19 subunit of the B-cell receptor. Our data also suggest that PI3K p85 subunit binds to the BCAP via SH3 domains forming an inactive complex that is activated subsequently by sequential binding with the SH2 domains. Taken together our results indicate that BCAP is a complex hub that processes signals from multiple pathways in diverse immune system cells.
HostingRepository	PRIDE
AnnounceDate	2019-09-16
AnnouncementXML	Submission_2019-09-16_00:47:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sven Eyckerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-27 01:22:12	ID requested
⏵ 1	2019-09-16 00:47:49	announced
2	2019-11-12 02:07:17	announced	2019-11-12: Updated publication reference for PubMed record(s): 31527084.
3	2024-10-22 04:06:10	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: human cell line HEK293T BCAP, phosphoinositide 3-kinase, Toll-like receptors, SH2 domains, SH3 domains, phosphorylation

Sven Eyckerman
contact affiliation	VIB Center for Medical Biotechnology Ghent University Dept. of Biomolecular Medicine
contact email	sven.eyckerman@vib-ugent.be
lab head
Sven Eyckerman
contact affiliation	VIB Center for Medical Biotechnology
contact email	sven.eyckerman@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014410

PRIDE project URI

• PRIDE
  1. PXD014410
     1. Label: PRIDE project
     2. Name: Phosphorylation of the multi functional signal transducer B-cell adaptor protein (BCAP) facilitates recruitment of multiple SH2/SH3 proteins including Grb2.