PXD014405-3

PXD014405 is an original dataset announced via ProteomeXchange.

Title	Yme1l substrate quantification under Leucine and Glutamine starvation as well as Torin treatment
Description	Metabolic reprogramming of mitochondria occurs during development, cell differentiation and in cancer and is coupled to changes in mitochondrial mass and shape. Here, we demonstrate that the i-AAA protease YME1L rewires the proteome of pre-existing mitochondria in response to hypoxia or nutrient starvation. Inhibition of mTORC1 induces a lipid signalling cascade via the phosphatidic acid phosphatase LIPIN1, which decreases phosphatidylethanolamine levels in mitochondrial membranes and promotes proteolysis. YME1L degrades mitochondrial protein translocases, lipid transfer proteins and metabolic enzymes to acutely limit mitochondrial biogenesis and support cell growth. YME1L-mediated mitochondrial reshaping ensures spheroid and xenograft growth of pancreatic ductal adenocarcinoma (PDAC) cells. Similar mitochondrial proteome changes occur in tumor tissues of PDAC patients, suggesting that YME1L is relevant to the pathophysiology of specific solid tumors. Our results identify the mTORC1-LIPIN1-YME1L axis as a novel post-translational regulator of mitochondrial proteostasis at the interface
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:59.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hendrik Nolte
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-27 00:51:57	ID requested
1	2019-08-21 02:36:37	announced
2	2019-11-18 01:05:10	announced	2019-11-18: Updated publication reference for PubMed record(s): 31695197.
⏵ 3	2024-10-22 04:06:06	announced	2024-10-22: Updated project metadata.

MacVicar T, Ohba Y, Nolte H, Mayer FC, Tatsuta T, Sprenger HG, Lindner B, Zhao Y, Li J, Bruns C, Kr, ü, ger M, Habich M, Riemer J, Schwarzer R, Pasparakis M, Henschke S, Br, ü, ning JC, Zamboni N, Langer T, Lipid signalling drives proteolytic rewiring of mitochondria by YME1L. Nature, 575(7782):361-365(2019) [pubmed]

10.1038/s41586-019-1738-6;

curator keyword: Biological

submitter keyword: DIA,MEF cells, YME1L, LC-MS/MS

Thomas Langer
contact affiliation	Max Planck Institute for Biology of Ageing Joseph-Stelzmann-Str. 9b D-50931 Cologne, Germany
contact email	TLanger@age.mpg.de
lab head
Hendrik Nolte
contact affiliation	Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact email	h.nolte@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD014405

PRIDE project URI

• PRIDE
  1. PXD014405
     1. Label: PRIDE project
     2. Name: Yme1l substrate quantification under Leucine and Glutamine starvation as well as Torin treatment