PXD014368-1

PXD014368 is an original dataset announced via ProteomeXchange.

Title	Discovery of TBC1D7 as a potential driver for melanoma cell invasion.
Description	Melanoma is a common type of cancer, and metastasis remains the leading cause for mortality in melanoma patients. In this study, we utilized an unbiased mass spectrometry-based quantitative proteomic method to assess, at the global proteome scale, differential protein expression in a matched pair of primary/metastatic melanoma cell lines derived from the same patient, i.e. WM-115/WM-266-4. We found that TBC1D7 is overexpressed in metastatic (WM-266-4) relative to primary (WM-115) melanoma cells. We also observed that elevated expression of TBC1D7 promotes melanoma metastasis in vitro. Bioinformatic analyses of The Cancer Genome Atlas (TCGA) data suggested that higher mRNA expression levels of TBC1D7 predict poorer survival in melanoma patients. Furthermore, we showed that TBC1D7 promotes invasion of cultured melanoma cells in vitro, at least in part, through modulating the expression levels and activities of matrix metalloproteinases 2 and 9 (MMP2 and MMP9). Together, the results from the present study support TBC1D7 as a potential driver for melanoma metastasis.
HostingRepository	PRIDE
AnnounceDate	2022-08-15
AnnouncementXML	Submission_2022-08-14_18:32:32.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianyu Qi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-25 01:44:26	ID requested
⏵ 1	2022-08-14 18:32:32	announced
2	2023-11-14 08:50:35	announced	2023-11-14: Updated project metadata.

10.1002/PMIC.201900347;

submitter keyword: quantitative proteomics, SILAC, mass spectrometry, melanoma metastasis, TBC1D7, TBC domain-containing proteins

Yinsheng Wang
contact affiliation	Professor of Chemistry University of California Riverside Riverside, CA 92521-0403
contact email	yinsheng@ucr.edu
lab head
Tianyu Qi
contact affiliation	UC RIVERSIDE
contact email	tqi001@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD014368

PRIDE project URI

• PRIDE
  1. PXD014368
     1. Label: PRIDE project
     2. Name: Discovery of TBC1D7 as a potential driver for melanoma cell invasion.