PXD014347 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mouse Mammary Gland Morphogenic Differences with BAD 3SA Mutant |
Description | The identification of genes driving organ development is central to understanding which signaling pathways drive the pathogenesis of various diseases including cancer. This dataset depicts the proteomic changes observed in C57BL/6J mice expressing wild-type or 3SA-phospho mutant versions of the Bcl-2-associated death promoter, BAD. This data shows that BAD regulates postnatal mammary gland morphogenesis in puberty. Three conserved serine residues on BAD are co-ordinately phosphorylated to regulate its activity. Non-phosphorylated BAD mutant delayed pubertal ductal elongation. This defect was specific to the epithelial compartment as transplant and ex vivo organoid assays of mutant epithelium recapitulated decreased tubule migration. Proteomic signature between BAD+/+ and phosphomutant BAD-3SA mammary glands identified differences in actin-binding and focal adhesion components. Mechanistically, non-phosphorylated BAD impedes protein translation, specifically in protrusions, through aberrant hypophosphorylated 4E-BP1. These findings reveal a critical enhancement of localized translation for efficient pubertal-mammary-gland morphogenesis and identifies BAD as a novel regulator of this process. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-29 |
AnnouncementXML | Submission_2021-03-29_03:13:56.452.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Kramer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-24 02:33:50 | ID requested | |
⏵ 1 | 2021-03-29 03:13:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mouse, Mammary Gland, Breast, LC-MS/MS, BAD, Mammary Morphogenesis, Breast Cancer |
Contact List
Richard P Fahlman |
contact affiliation | Professor, Department of Biochemistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada |
contact email | rfahlman@ualberta.ca |
lab head | |
David Kramer |
contact affiliation | University of Alberta Department of Biochemistry |
contact email | dakramer@ualberta.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014347
- Label: PRIDE project
- Name: Mouse Mammary Gland Morphogenic Differences with BAD 3SA Mutant