PXD014339-2

PXD014339 is an original dataset announced via ProteomeXchange.

Title	CHD7- and HDAC1/2-dependent chromatin remodeling activities cooperate to promote DNA double-strand break repair
Description	The DNA double-strand break (DSB) response is a multi-step process that requires chromatin reorganization at each stage. 53BP1 is a nodal point in this response as it directs chromatin-based DSB repair. However, how chromatin reorganization impacts DSB repair upstream of 53BP1 is not fully understood. Here we reveal that Chromodomain Helicase DNA Binding Protein 7 (CHD7) rapidly recruits to damaged chromatin in a poly(ADP-ribose) polymerase 1 (PARP1)-dependent manner. CHD7 facilitates chromatin relaxation and the loading of canonical non-homologous end-joining (cNHEJ) factors, while restraining 53BP1 accumulation at DSBs. Moreover, CHD7 physically associates with HDAC1/2, thereby recruiting this complex to DNA breaks independently of its chromatin remodeling activity. HDAC1/2 promote histone H4 de-acetylation and chromatin re-compaction to prevent supraphysiological retention of cNHEJ factors at DBSs. Thus, the cooperation between distinct yet coordinated chromatin remodeling activities driven by CHD7 and HDAC1/2 ensures proper assembly dynamics of the cNHEJ machinery and efficient DSB repair.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:25.953.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alfred Vertegaal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-21 03:50:44	ID requested
1	2020-08-27 23:02:35	announced
⏵ 2	2024-10-22 05:11:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DNA double-strand break (DSB) repair, non-homologous end-joining (NHEJ), HDAC1/2, chromatin remodeling, CHD7

Alfred C.O. Vertegaal
contact affiliation	Cell and Chemical Biology Leiden University Medical Center Leiden, the Netherlands
contact email	vertegaal@lumc.nl
lab head
Alfred Vertegaal
contact affiliation	Leiden University Medical Center
contact email	vertegaal@lumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014339
     1. Label: PRIDE project
     2. Name: CHD7- and HDAC1/2-dependent chromatin remodeling activities cooperate to promote DNA double-strand break repair