PXD014323-1

PXD014323 is an original dataset announced via ProteomeXchange.

Title	Host cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.
Description	We previously reported that the utilization of host cholesterol is essential for mycobacterial persistence. In this study, we demonstrated that cholesterol-induced activation of a ribonuclease toxin (VapC12) inhibits translation by targeting proT tRNA in Mtb. The resulting cholesterol-specific growth modulation increases the frequency of the generation of persisters in a heterogeneous Mtb population. A vapC12-null mutant strain (ΔvapC12) failed to persist and demonstrated a hypervirulent phenotype in a guinea pig model of Mtb infection. This is the first study to identify a novel strategy through which cholesterol-specific activation of a toxin–antitoxin (TA) module in Mtb enhances persister formation during infection. In addition to identifying the mechanism, the study provides opportunity for targeting persisters, a new paradigm facilitating tuberculosis drug development.
HostingRepository	PRIDE
AnnounceDate	2020-10-14
AnnouncementXML	Submission_2020-10-14_05:28:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renu Goel
SpeciesList	scientific name: Mycobacterium tuberculosis M24; NCBI TaxID: 1268063;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-20 04:14:55	ID requested
⏵ 1	2020-10-14 05:28:58	announced

Dataset with its publication pending

submitter keyword: TripleTOF5600, mass-spectrometer, proteomics, mycobacterium

Dr Nisheeth Agrawal
contact affiliation	Translational Health Science and Technology Institute
contact email	nisheeth@thsti.res.in
lab head
Renu Goel
contact affiliation	Translational Health Science and technology Institute
contact email	drrenugoel@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014323
     1. Label: PRIDE project
     2. Name: Host cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.