PXD014322 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic screening of exercise-like treatments reveals drug interactions regulating protein secretion |
Description | Exercise triggers skeletal muscle signalling pathways that modulate the release of circulating factors to cause systemic health benefits. Understanding these mechanisms could lead to better strategies for treating cardiometabolic diseases. We previously showed that acute exercise induces >1000 changes in protein phosphorylation in human muscle. Here we employed a strategy to deconvolute this network by analysing phosphoproteomes of rat L6 myotubes treated with small molecules that mimic different aspects of exercise signalling. Bioinformatics suggested that combining β-adrenergic and calcium agonists would yield a phosphoproteome most closely resembling exercise. Experimental analysis supported this but also revealed a surprising divergence in signalling from that observed with either drug alone. Dual stimulation promoted multisite phosphorylation of SERBP1, a regulator of Serpine1 mRNA stability, a pro-thrombotic fibrotic secreted protein. Secretomic analysis of L6 myotubes treated with β-adrenergic and calcium agonists revealed a significant decrease in SERPINE1 secretion and other deleterious secretory factors. This provides a novel approach to dissect the beneficial effects of exercise and demonstrates an underappreciated effect of exercise to reduce the circulating levels of certain factors, providing new insights into exercise benefits and their therapeutic potential. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-26 |
AnnouncementXML | Submission_2019-09-25_21:33:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Parker |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-20 03:20:52 | ID requested | |
⏵ 1 | 2019-09-25 21:33:39 | announced | |
2 | 2019-11-11 00:34:41 | announced | 2019-11-11: Updated publication reference for PubMed record(s): 31693893. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphoproteomics, myotubes, exercise |
Contact List
Benjamin Parker |
contact affiliation | the University of Sydney |
contact email | benjamin.parker@sydney.edu.au |
lab head | |
Benjamin Parker |
contact affiliation | The University of Sydney |
contact email | benjamin.parker@sydney.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014322
- Label: PRIDE project
- Name: Phosphoproteomic screening of exercise-like treatments reveals drug interactions regulating protein secretion