PXD014315-2

PXD014315 is an original dataset announced via ProteomeXchange.

Title	Active thrombin is produced by intestinal epithelium to control mucosal biofilm
Description	Healthy human and mouse colon epithelium is a major source of active thrombin, released in lumen. Using germ-free animals, we demonstrated that mucosal thrombin was directly regulated by the presence of commensal microbiota. Specific inhibition of lumenal thrombin activity caused macro-, microscopic damage and transcriptomic alterations of genes involved in host-microbiota interactions. Further, lumenal thrombin inhibition impaired the spatial segregation of microbiota biofilms, allowing bacteria to invade the mucus layer and to translocate across the epithelium. Thrombin proteolyzed the biofilm matrix of reconstituted mucosa-associated human microbiota. We demonstrated a previously unknown physiological role for epithelial thrombin that constrains biofilms at mucosal surfaces. We report that lung, bladder and skin epithelia also expressed thrombin, suggesting that this role may be applicable to other host-microbiome surfaces. Our discovery points route to new therapies targeting biofilms, important for a broad range of disorders, in the gut, and beyond.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:54.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-20 02:04:13	ID requested
1	2019-06-24 07:04:13	announced
⏵ 2	2024-10-22 04:08:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: gut physiology,thrombin, microbial biofilm, microbiota spatial organization, epithelia

Antoine Dufour
contact affiliation	University of Calgary
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD014315

PRIDE project URI

• PRIDE
  1. PXD014315
     1. Label: PRIDE project
     2. Name: Active thrombin is produced by intestinal epithelium to control mucosal biofilm