PXD014307 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | UBR5 in B cell development TMT |
Description | Coordination of a number of molecular mechanisms including transcription, alternative splicing, and class switch recombination are required to facilitate development, activation, and survival of B cells. Disruption of these pathways can result in malignant transformation. Recently, next generation sequencing has identified a number of novel mutations in mantle cell lymphoma (MCL) patients including the ubiquitin E3 ligase UBR5. Approximately 18% of MCL patients were found to have mutations in UBR5 with the majority of mutations within the HECT domain of the protein which can accept and transfer ubiquitin molecules to the substrate. Determining if UBR5 controls the maturation of B cells is important to fully understand malignant transformation to MCL. To elucidate the role of UBR5 in B cell maturation and activation we generated a conditional mutant disrupting UBR5’s C-terminal HECT domain. Loss of the UBR5 HECT domain leads to a block in maturation of B cells in the spleen and up-regulation of proteins associated with mRNA splicing via the spliceosome. Our studies reveal a novel role of UBR5 in B cell maturation by regulating alternative splicing of key transcripts during B cell development and suggests UBR5 mutations may promote mantle cell lymphoma initiation. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-19 |
AnnouncementXML | Submission_2020-12-01_03:55:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Samantha Swenson |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; ubiquitination signature dipeptidyl lysine; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-20 02:00:48 | ID requested | |
1 | 2020-11-19 06:37:30 | announced | |
⏵ 2 | 2020-12-01 03:55:09 | announced | 2020-12-01: Updated publication reference for PubMed record(s): 32325489.
2020-12-01: Updated publication reference for DOI(s): 10.1182/blood.2019002102. |
3 | 2023-11-14 08:50:18 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.1182/blood.2019002102; |
Swenson SA, Gilbreath TJ, Vahle H, Hynes-Smith RW, Graham JH, Law HC, Amador C, Woods NT, Green MR, Buckley SM, UBR5 HECT domain mutations identified in mantle cell lymphoma control maturation of B cells. Blood, 136(3):299-312(2020) [pubmed] |
Keyword List
submitter keyword: TMT UBR5 Bcells Spleen Mouse |
Contact List
Shannon Mychel Buckley |
contact affiliation | Department of Genetics, Cell Bioliogy, & Anatomy Buckley Lab University of Nebraska Medical Center Omaha, Nebraska United States of America |
contact email | shannon.buckley@unmc.edu |
lab head | |
Samantha Swenson |
contact affiliation | University of Nebraska Medical Center |
contact email | samantha.swenson@unmc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014307
- Label: PRIDE project
- Name: UBR5 in B cell development TMT