PXD014288-2

PXD014288 is an original dataset announced via ProteomeXchange.

Title	Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
Description	1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. The results show that short digestion times apparently do not affect the number of identified proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:50.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-18 07:11:33	ID requested
1	2019-11-11 18:12:32	announced
⏵ 2	2024-10-22 04:56:50	announced	2024-10-22: Updated project metadata.

Wi, ś, niewski JR, Zettl K, Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation. Data Brief, 26():104530(2019) [pubmed]

10.1016/j.dib.2019.104530;

submitter keyword: FASP, Sample preparation

Jacek R Wisniewski
contact affiliation	Max Planck Institute of Biochemistry
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014288

PRIDE project URI

• PRIDE
  1. PXD014288
     1. Label: PRIDE project
     2. Name: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation