PXD014280-1

PXD014280 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Profiling the surfaceome identifies therapeutic targets for cells with hyperactive mTORC1 signaling
Description	Aberrantly high mTORC1 signaling is a known driver of many cancers and human disorders, yet pharmacological inhibition of mTORC1 rarely confers durable clinical responses. To explore alternative therapeutic strategies, herein we conducted a proteomics survey to identify cell surface proteins upregulated by mTORC1. A comparison of the surfaceome from Tsc1-/- versus Tsc1+/+ mouse embryonic fibroblasts revealed 59 proteins predicted to be significantly overexpressed in Tsc1-/- cells. Further validation of the data in multiple mouse and human cell lines showed that mTORC1 signaling most dramatically induced the expression of the proteases neprilysin (NEP/CD10) and aminopeptidase N (APN/CD13). Functional studies showed that constitutive mTORC1 signaling sensitized cells to genetic ablation of NEP and APN, as well as the biochemical inhibition of APN. In summary, these data show that mTORC1 signaling plays a significant role in the constitution of the surfaceome, which in turn may present novel therapeutic strategies.
HostingRepository	MassIVE
AnnounceDate	2020-01-15
AnnouncementXML	Submission_2020-01-15_11:28:24.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Leung
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; deglycosylated asparagine; 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-17 19:16:56	ID requested
⏵ 1	2020-01-15 11:28:25	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SILAC, TSC, mTORC1 singaling, Drug target

submitter keyword: SILAC, TSC, mTORC1 singaling, Drug target

Contact List

Kevin K Leung
contact affiliation	UCSF
contact email	kevin.leung@ucsf.edu
lab head
Kevin Leung
contact affiliation	UCSF
contact email	kevin.leung@ucsf.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083988/