PXD014274 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and unbiased post-translational modification profiling of amyloid plaques and surrounding tissue in a transgenic mouse model of Alzheimer’s disease |
Description | Amyloid plaques (Aβ plaques) are one of the hallmarks of Alzheimer’s disease (AD). The main constituent of Aβ plaques is beta-amyloid peptides but a complex interplay of other infiltrating proteins also co-localizes. We focused on proteomic differences between Aβ plaques and adjacent control tissue in the transgenic mouse model of AD (APPPS1-21) and in similar regions from non-transgenic littermates. A microproteomic strategy included isolation of regions of interest by laser capture microdissection and analyzed by label-free liquid chromatography mass spectrometry. An in-solution digest protocol with a buffer containing an acid-labile surfactant was used to increase protein solubilization and protease efficiency. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-25 |
AnnouncementXML | Submission_2021-03-25_02:30:13.442.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Allan Stensballe |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-17 03:49:26 | ID requested | |
⏵ 1 | 2021-03-25 02:30:13 | announced | |
2 | 2021-03-25 05:23:32 | announced | 2021-03-25: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Amyloid plaque, laser capture microdissection, Alzheimer’s disease, mass spectrometry, post transcriptional modifications |
Contact List
Allan Stensballe |
contact affiliation | Laboratory for Medical Massspectrometry; Aalgorg University |
contact email | as@hst.aau.dk |
lab head | |
Allan Stensballe |
contact affiliation | Aalborg University |
contact email | as@hst.aau.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014274
- Label: PRIDE project
- Name: Proteomic and unbiased post-translational modification profiling of amyloid plaques and surrounding tissue in a transgenic mouse model of Alzheimer’s disease