PXD014256 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Embryonic day 15 chick utricle single cell analysis |
Description | Single-cell proteomics can reveal the changing protein composition of differentiating cells. We used shotgun mass spectrometry to determine the abundant proteins present in single or small pools of subpicoliter-sized cells from the embryonic day 15 (E15) utricle of the chicken inner ear, when many hair cells are differentiating from progenitor (supporting) cells. The actin monomer binding protein thymosin β4 (TMSB4X) was present in E15 progenitor cells at nearly equimolar levels relative to actin, but dropped to one-tenth that value in hair cells, with little change in total actin. Single-cell RNA-seq analysis of E15 utricle cells showed that TMSB4X transcripts fell in abundance once hair-cell differentiation initiated. These results suggest that most actin is sequestered in progenitor cells, but upon differentiation to hair cells, actin is released, permitting assembly of the sensory hair bundle. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-11_23:47:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Barr-Gillespie |
SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-14 01:30:08 | ID requested | |
⏵ 1 | 2019-11-11 23:47:54 | announced | |
2 | 2020-01-14 06:22:46 | announced | 2020-01-14: Updated publication reference for PubMed record(s): 31682227. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ear, utricle, single cell, actin, thymosin beta, hair cell, supporting cell |
Contact List
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 USA |
contact email | gillespp@ohsu.edu |
lab head | |
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center |
contact email | gillespp@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014256 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014256
- Label: PRIDE project
- Name: Embryonic day 15 chick utricle single cell analysis