<<< Full experiment listing

PXD014256-1

PXD014256 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEmbryonic day 15 chick utricle single cell analysis
DescriptionSingle-cell proteomics can reveal the changing protein composition of differentiating cells. We used shotgun mass spectrometry to determine the abundant proteins present in single or small pools of subpicoliter-sized cells from the embryonic day 15 (E15) utricle of the chicken inner ear, when many hair cells are differentiating from progenitor (supporting) cells. The actin monomer binding protein thymosin β4 (TMSB4X) was present in E15 progenitor cells at nearly equimolar levels relative to actin, but dropped to one-tenth that value in hair cells, with little change in total actin. Single-cell RNA-seq analysis of E15 utricle cells showed that TMSB4X transcripts fell in abundance once hair-cell differentiation initiated. These results suggest that most actin is sequestered in progenitor cells, but upon differentiation to hair cells, actin is released, permitting assembly of the sensory hair bundle.
HostingRepositoryPRIDE
AnnounceDate2019-11-12
AnnouncementXMLSubmission_2019-11-11_23:47:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Barr-Gillespie
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-14 01:30:08ID requested
12019-11-11 23:47:54announced
22020-01-14 06:22:46announced2020-01-14: Updated publication reference for PubMed record(s): 31682227.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ear, utricle, single cell, actin, thymosin beta, hair cell, supporting cell
Contact List
Peter Barr-Gillespie
contact affiliationOregon Hearing Research Center Oregon Health & Science University Portland OR 97239 USA
contact emailgillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliationOregon Hearing Research Center
contact emailgillespp@ohsu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014256
PRIDE project URI
Repository Record List
[ + ]