PXD014241-1

PXD014241 is an original dataset announced via ProteomeXchange.

Title	MFSD1 KO Liver - Lysosomes are a major site of intracellular acidic hydrolase-mediated proteolysis and cellular degradation
Description	Lysosomes are a major site of intracellular acidic hydrolase-mediated proteolysis and cellular degradation. The export of low-molecular catabolic end products from the lysosomal lumen to the cytosol is facilitated by polytopic transmembrane proteins which mediate secondary active or passive transport. One of these proteins is MFSD1, a so far uncharacterized lysosomal 12 transmembrane domain-containing family member of the major facilitator superfamily. MFSD1 is, uncommon for a lysosomal membrane protein, not N-glycosylated. It contains a dileucine-based sorting motif that is essential for its transport to lysosomes. Lysosomes from mouse liver of MFSD1 knock-out mice were isolated and analyzed by TMT labelling, as well as whole liver protein extracts, both in comparison to wildtype controls.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:10:54.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Thelen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-13 01:08:41	ID requested
⏵ 1	2021-03-04 12:10:54	announced
2	2024-10-22 04:54:08	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: MFSD1, lysosome, liver

Melanie Thelen
contact affiliation	University Hospital Bonn Institute for Biochemistry and Molecular Biology Nussallee 11 53115 Bonn Germany
contact email	mthelen@uni-bonn.de
lab head
Melanie Thelen
contact affiliation	Institute for Biochemistry and Molecular Biology, University Bonn, Germany
contact email	mthelen@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014241

PRIDE project URI

• PRIDE
  1. PXD014241
     1. Label: PRIDE project
     2. Name: MFSD1 KO Liver - Lysosomes are a major site of intracellular acidic hydrolase-mediated proteolysis and cellular degradation