PXD014238 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteome analysis of the Arabidopsis thaliana N-degron pathway E3 ligase mutant, prt1-1. |
Description | The N-degron pathway relates the half-life of a protein to its amino terminal (Nt) residue. Following cleavage of proteins by endopeptidases, new N-t amino acids are revealed and can be recognised by different E3 ligase enzymes that target the substrate proteins for degradation via the proteasome. Studies with artificial proteins have shown that PRT1 is an Arabidopsis thaliana E3 ligase with specificity for aromatic N-termini (phenylalanine, tyrosine, tryptophan), but very little is known about its physiological roles and endogenous substrates. To gain insight into the impact of PRT1 on the proteome, we conducted a quantitative proteomic analysis, comparing leaf proteins from the prt1-1 loss of function mutant with those from wild type plants. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-01-09_06:42:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hongtao Zhang |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-12 08:56:34 | ID requested | |
⏵ 1 | 2020-01-09 06:42:32 | announced | |
Publication List
Till CJ, Vicente J, Zhang H, Oszvald M, Deery MJ, Pastor V, Lilley KS, Ray RV, Theodoulou FL, Holdsworth MJ, N-recognin E3 ligase PROTEOLYSIS1 influences the immune response. Plant Direct, 3(12):e00194(2019) [pubmed] |
Keyword List
submitter keyword: Arabidopsis, N-degron pathway PRT1 leaves TMT- labelling |
Contact List
Hongtao Zhang |
contact affiliation | Department of Plant Sciences Rothamsted Research, West Common, Harpenden, Herts, AL5 2JQ, UK |
contact email | hongtao.zhang@rothamsted.ac.uk |
lab head | |
Hongtao Zhang |
contact affiliation | Plant Sciences Department, Rothamsted Research, Harpenden, AL5 2JQ, UK |
contact email | hongtao.zhang@rothamsted.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014238
- Label: PRIDE project
- Name: Quantitative proteome analysis of the Arabidopsis thaliana N-degron pathway E3 ligase mutant, prt1-1.